Zhu Yue, Shi Xi, Zhang Shanshan, Cui Hongyin, Wu Baohua
Department of Gynecology, YiWu Central Hospital, Jinhua, 322000, China.
Department of Gynecology and Obstetrics, DAYE Maternity and Child Health Hospital, Huangshi, 435100, China.
Discov Oncol. 2025 May 23;16(1):879. doi: 10.1007/s12672-025-02670-5.
To investigate the prognostic value of circ_0000043 in cervical cancer (CC) and its involvement in the modulation of patients' prognostic progression.
Clinical data of 136 CC patients were included, postoperative CC and adjacent normal tissues were stored at -80 °C, and patients were followed up for 5 years for prognosis. RT-qPCR detected circ_ 0000043 and miR-590-5p expression, Kaplan-Meier curves recorded for prognostic survival, multivariate cox analysis of patients' prognostic risk factors. Moreover, CCK8 assessed cell proliferation, Transwell detected migration and invasion. Dual luciferase reporter assay and RIP examined the interactions between circ_ 0000043 and miR-590-5p.
circ_ 0000043 was down-regulated in CC patients, and patients with low expression of circ_ 0000043 had worse survival. Low expression of circ_0000043, lymph node metastasis, and FIGO were all unfavorable factors threatening the prognosis of CC. circ_0000043 overexpression markedly inhibited the proliferation, migration, and invasion of cancer cells. miR-590-5p is a direct target miRNA of circ_0000043. miR-590-5p mimic prominently resisted the altered cellular functions induced by circ_0000043.
Patients with low circ_0000043 expression have poor prognosis as it fuels CC progression by boosting cancer cell proliferation, migration, and invasion via miR-590-5p. Our study provides new ideas for the prognosis and treatment of CC.
探讨circ_0000043在宫颈癌（CC）中的预后价值及其在调节患者预后进展中的作用。
纳入136例CC患者的临床资料，将术后CC组织及相邻正常组织储存于-80°C，对患者进行5年预后随访。RT-qPCR检测circ_0000043和miR-590-5p表达，绘制Kaplan-Meier曲线记录预后生存情况，对患者预后危险因素进行多因素cox分析。此外，CCK8法评估细胞增殖，Transwell法检测迁移和侵袭能力。双荧光素酶报告基因检测和RIP实验检测circ_0000043与miR-590-5p之间的相互作用。
CC患者中circ_0000043表达下调，circ_0000043低表达患者的生存情况较差。circ_0000043低表达、淋巴结转移和国际妇产科联盟（FIGO）分期均是威胁CC预后的不利因素。circ_0000043过表达明显抑制癌细胞的增殖、迁移和侵袭。miR-590-5p是circ_0000043的直接靶向miRNA。miR-590-5p模拟物显著抵抗circ_0000043诱导的细胞功能改变。
circ_0000043低表达的患者预后较差，因为它通过miR-590-5p促进癌细胞增殖、迁移和侵袭，从而推动CC进展。我们的研究为CC的预后和治疗提供了新思路。
