碳水化合物的荧光标记及液相色谱分析。使用甘露糖苷贮积症寡糖对衍生物进行比较。

Fluorescent labeling of carbohydrates and analysis by liquid chromatography. Comparison of derivatives using mannosidosis oligosaccharides.

作者信息

Coles E, Reinhold V N, Carr S A

出版信息

Carbohydr Res. 1985 Jun 15;139:1-11. doi: 10.1016/0008-6215(85)90001-1.

Abstract

To enhance resolution and detectability of carbohydrates by liquid chromatography, two fluorescent labels, introduced into oligosaccharides by reductive amination, were compared by use of standard sugars and a complex, biological sample of D-mannose oligomers obtained from the urine of a mannosidosis patient. Both labels, 2-aminopyridine and 7-amino-1-naphthol, improved the chromatographic efficiency and detection sensitivity. However, reductive amination with the pyridinylamine derivative was incomplete. The Schiff-base intermediates left in the mixtures were only partially resolved by chromatography and complicate the patterns. In contrast, the naphtholamine derivatives were completely reduced and, in addition, possess enhanced fluorescence.

摘要

为了通过液相色谱提高碳水化合物的分辨率和可检测性，通过还原胺化将两种荧光标记引入寡糖中，利用标准糖以及从一名甘露糖苷贮积症患者尿液中获得的复杂的D-甘露糖寡聚物生物样品对这两种标记进行了比较。两种标记，即2-氨基吡啶和7-氨基-1-萘酚，均提高了色谱效率和检测灵敏度。然而，用吡啶胺衍生物进行的还原胺化并不完全。混合物中残留的席夫碱中间体仅通过色谱部分分离，使图谱复杂化。相比之下，萘胺衍生物被完全还原，此外，还具有增强的荧光。