一种纯化细胞质副粘病毒核衣壳的快速方法。
A rapid method for the purification of cytoplasmic paramyxoviral nucleocapsids.
作者信息
Robbins S J, Abbott-Smith P M
出版信息
J Virol Methods. 1985 Jul;11(3):253-7. doi: 10.1016/0166-0934(85)90114-4.
PMID:4040923
Abstract
A rapid method for the purification of cytoplasmic paramyxoviral nucleocapsids using a tabletop ultracentrifuge is described. The method yields materials equivalent in purity to materials purified by previously published procedures but requires only a fraction of the time (65 min vs 7-41.5 h). When used in conjunction with modern hypersensitive analytical techniques, the procedure provides equivalent or greater amounts of nucleocapsid for subsequent analysis or study.
摘要
本文描述了一种使用台式超速离心机快速纯化细胞质副粘病毒核衣壳的方法。该方法所获得材料的纯度与先前发表的方法纯化的材料相当，但所需时间仅为其一小部分（65分钟对7 - 41.5小时）。当与现代超灵敏分析技术结合使用时，该程序可为后续分析或研究提供等量或更多的核衣壳。
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