Moore P M, Hayes E C, Miller S E, Wright L L, Machamer C E, Zweerink H J
Infect Immun. 1978 Jun;20(3):842-6. doi: 10.1128/iai.20.3.842-846.1978.
Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbits against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.
从109个感染麻疹病毒的细胞中纯化出了接近1毫克的核衣壳。它们含有一种分子量为59,000的多肽。用纯化的核衣壳免疫兔子制备抗血清，并用于竞争性放射免疫测定。由于纯化的核衣壳不稳定，不适合用于此类测定。取而代之的是，使用来自持续感染麻疹病毒的HEp-2细胞并在体外用[35S]甲硫氨酸标记的部分纯化核衣壳作为放射性抗原的来源。由此开发的放射免疫测定法可检测感染细胞中少于5纳克的核衣壳。
