Yang Yong-Qiang, Li Xu, Wang Zhen-Zhi, Huang Xiao-Yan, Zeng Du-Wen, Zhao Xin-Qing, Liu Zhi-Qiang, Zhang Feng-Li
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; School of Life and Health Sciences, Hainan University, Haikou 570228, China.
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Bioresour Technol. 2025 Oct;433:132649. doi: 10.1016/j.biortech.2025.132649. Epub 2025 May 21.
With the world population continuously increasing, the protein demand will double by 2050. Single cell protein (SCP) derived from lignocellulosic biomass offers a sustainable solution. Many inhibitors are produced during the pretreatment process of lignocellulosic biomass. Inhibitor-rich hydrolysates limit microorganisms cell growth and SCP yields. In this work, we report a co-culture consortium of Kodamaea ohmeri SSK (pentose-utilizing yeast) and Lactococcus lactis LX (probiotic bacterium) that efficiently converts real corn straw hydrolysate into SCP. K. ohmeri SSK can tolerate inhibitors such as furfural, 5-hydroxymethylfurfural (5-HMF), and acetic acid and consume glucose, xylose, and arabinose in real hydrolysate. L. lactis LX showed less growth in monoculture than that of co-culture. The total amino acid content from co-cultured K. ohmeri SSK and L. lactis LX was increased to 331.42 mg/g crude protein, but that of monocultured K. ohmeri SSK was 309.89 mg/g crude protein containing 17 amino acids. This work demonstrates a symbiotic microbial platform can produce SCP from non-detoxified lignocellulosic biomass. The co-culture robust inhibitor tolerance and balanced amino acid profile highlight its potential for industrial-scale protein production. These results will represent an attractive choice cell factory for lignocellulosic substrate utilization and provide a platform for biomass conversion to SCP.
随着世界人口持续增长，到2050年蛋白质需求将翻倍。源自木质纤维素生物质的单细胞蛋白（SCP）提供了一种可持续的解决方案。在木质纤维素生物质的预处理过程中会产生许多抑制剂。富含抑制剂的水解产物会限制微生物细胞生长和SCP产量。在这项工作中，我们报道了一种由欧默柯达酵母SSK（戊糖利用酵母）和乳酸乳球菌LX（益生菌）组成的共培养联合体，它能有效地将实际玉米秸秆水解产物转化为SCP。欧默柯达酵母SSK能够耐受诸如糠醛、5-羟甲基糠醛（5-HMF）和乙酸等抑制剂，并消耗实际水解产物中的葡萄糖、木糖和阿拉伯糖。乳酸乳球菌LX在单培养中的生长比共培养中的生长要少。共培养的欧默柯达酵母SSK和乳酸乳球菌LX的总氨基酸含量增加到331.42毫克/克粗蛋白，而单培养的欧默柯达酵母SSK的总氨基酸含量为309.89毫克/克粗蛋白，含有17种氨基酸。这项工作表明，一个共生微生物平台可以从不解毒的木质纤维素生物质中生产SCP。共培养强大的抑制剂耐受性和平衡的氨基酸谱突出了其在工业规模蛋白质生产中的潜力。这些结果将为木质纤维素底物利用提供一个有吸引力的细胞工厂选择，并为生物质转化为SCP提供一个平台。
