Hu Xinru, Zhong Zhen, Yu Longhui, Yang Qinru, Zhang Jinfeng, Wang Wenjing, Chen Peiqin, Li Jingen, Zheng Guodong
College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China.
Food Chem Toxicol. 2025 Sep;203:115557. doi: 10.1016/j.fct.2025.115557. Epub 2025 May 21.
Exhaustive fatigue (EF) has become a common concern, and using natural active ingredients as prebiotics has become prospective strategy for fatigue alleviation. Millettia speciosa Champ. root (MS) has anti-fatigue activity, but the relationship between MS anti-fatigue effect and gut microbiota remains unclear.
To investigate the relationship between anti-fatigue effect of MS extracts and gut microbiota.
MS was extracted with water and ethanol to obtain MS water extract (MSW) and MS ethanol extract (MSE). Sixty ICR mice were divided into six groups: Control, American ginseng, low-, high-doses MSW and low-, high-doses MSE for 28 days. Fatigue related biochemical indexes and gut microbiota of cecum contents were analyzed.
MSW and MSE effectively prolonged swimming time and climbing time in mice, decreased harmful metabolites, increased the glycogen content in the liver and muscle, and reduced oxidative stress damage. Furthermore, the dysbiosis of gut microbiota induced by EF was restored by MSW and MSE, including enriching beneficial bacteria and depleting harmful bacteria.
The results suggested that MS extracts may effectively alleviate EF though improving exercise endurance, reducing oxidative stress and regulating gut microbiota homeostasis in mice. Therefore, MS extracts may be considered as a prebiotic agent to improve EF.
力竭性疲劳已成为一个普遍关注的问题，使用天然活性成分作为益生元已成为缓解疲劳的前瞻性策略。牛大力根具有抗疲劳活性，但其抗疲劳作用与肠道微生物群之间的关系尚不清楚。
探讨牛大力提取物抗疲劳作用与肠道微生物群之间的关系。
用水和乙醇提取牛大力，得到牛大力水提取物（MSW）和牛大力乙醇提取物（MSE）。将60只ICR小鼠分为6组：对照组、人参组、低剂量和高剂量MSW组以及低剂量和高剂量MSE组，给药28天。分析疲劳相关生化指标和盲肠内容物的肠道微生物群。
MSW和MSE有效延长了小鼠的游泳时间和攀爬时间，减少了有害代谢产物，增加了肝脏和肌肉中的糖原含量，并减少了氧化应激损伤。此外，MSW和MSE恢复了由力竭性疲劳诱导的肠道微生物群失调，包括富集有益菌和减少有害菌。
结果表明，牛大力提取物可能通过提高运动耐力、减少氧化应激和调节小鼠肠道微生物群稳态来有效缓解力竭性疲劳。因此，牛大力提取物可被视为一种改善力竭性疲劳的益生元制剂。
