Potgieter H C, Spies J H, Klein T, Thierry M, van der Watt J J
J Recept Res. 1985;5(2-3):193-218. doi: 10.3109/10799898509041879.
Experimental conditions for the optimal measurement of estrogen (ER) and progesterone (PR) receptors in normal vervet monkey (Cercopithecus aethiops pygerythrus) uteri are described. The uteri of this primate were found to contain relatively high concentrations of both ER and PR. Levels of ER ranged from 151 to 822 femtomoles per mg protein (mean for group assayed is 327 +/- 165 femtomoles per mg protein). PR assays were performed on the same cytosols and the levels ranged from 444 to 2267 femtomoles per mg protein (mean of 1285 +/- 511 femtomoles per mg protein). Mean Kd values for the ER- and PR-ligand complexes were found to be 3.15 +/- 1.4 X 10(-10)M and 2.38 +/- 0.2 X 10(-9)M respectively, within the group analysed (n = 21). The ratio of PR to ER varied between 1.1 and 13.1 with a mean of 4.5 +/- 2.4. Ligand specificity studies revealed that [3H]-17 beta-estradiol binding to the ER could only be inhibited by estrogens or estrogen analogues. The PR however exhibited an affinity for a wider range of ligand types. In low ionic strength buffers both ER and PR sedimented as approximately 8S type molecules in the presence or absence of 10mM sodium molybdate. Both receptors dissociated into smaller components, following a short exposure to 0.4 M KCl and subsequent centrifugation in a gradient containing 0.4 M KCl.
本文描述了在正常黑长尾猴（赤猴亚种）子宫中对雌激素（ER）和孕激素（PR）受体进行最佳测量的实验条件。发现这种灵长类动物的子宫中ER和PR的浓度相对较高。ER水平范围为每毫克蛋白质151至822飞摩尔（所检测组的平均值为每毫克蛋白质327±165飞摩尔）。在相同的胞液中进行PR检测，其水平范围为每毫克蛋白质444至2267飞摩尔（平均值为1285±511飞摩尔每毫克蛋白质）。在所分析的组（n = 21）中，发现ER - 配体复合物和PR - 配体复合物的平均解离常数（Kd）值分别为3.15±1.4×10⁻¹⁰M和2.38±0.2×10⁻⁹M。PR与ER的比值在1.1至13.1之间变化，平均值为4.5±2.4。配体特异性研究表明，[³H] - 17β - 雌二醇与ER的结合仅能被雌激素或雌激素类似物抑制。然而，PR对更广泛的配体类型表现出亲和力。在低离子强度缓冲液中，无论是否存在10mM钼酸钠，ER和PR均以约8S型分子沉降。在短暂暴露于0.4M KCl并随后在含有0.4M KCl的梯度中离心后，两种受体均解离为较小的组分。
