γ-氨基丁酸对[³H]亮氨酸掺入大鼠输卵管核糖体系统无作用。

Lack of effect of GABA on [3H]leucine incorporation into a rat oviduct ribosomal system.

作者信息

Orensanz L M, Azuara C, Fernández I

出版信息

Neurochem Res. 1985 Jun;10(6):789-96. doi: 10.1007/BF00964536.

Abstract

A ribosomal system for [3H]leucine incorporation was isolated from the rat oviduct in order to study the possible effect of GABA on [3H]leucine incorporation during the estrous cycle. The system showed an absolute requirement for Mg2+ and about 50% dependence on an energy source. Optimal [3H]leucine incorporation occurred under 3-6 mM Mg2+ and 100 mM K+ and was higher in diestrous-1 than in estrous or proestrous. GABA (10 mM) had no effect on [3H]leucine incorporation in any of the three estrous phases studied.

摘要

为了研究γ-氨基丁酸（GABA）在发情周期中对[³H]亮氨酸掺入的可能影响，从大鼠输卵管中分离出了一个用于[³H]亮氨酸掺入的核糖体系统。该系统对Mg²⁺有绝对需求，并且约50%依赖于能量来源。在3 - 6 mM Mg²⁺和100 mM K⁺条件下发生最佳的[³H]亮氨酸掺入，且在间情期-1比发情期或发情前期更高。在所研究的三个发情阶段中的任何一个阶段，GABA（10 mM）对[³H]亮氨酸掺入均无影响。