Tao Winnie C W, Niermans Kelly, Weide Yoran B N, van Dongen Katja C W, Meijer Nathan, Hoek-van den Hil Elise F, van der Fels-Klerx H J
Wageningen Food Safety Research (WFSR), Wageningen University & Research, Akkermaalsbos 2, Wageningen, WB 6708, the Netherlands.
Wageningen Food Safety Research (WFSR), Wageningen University & Research, Akkermaalsbos 2, Wageningen, WB 6708, the Netherlands.
Environ Toxicol Pharmacol. 2025 Aug;117:104724. doi: 10.1016/j.etap.2025.104724. Epub 2025 May 22.
Aflatoxin B (AFB)-contaminated crops could serve as insect feed without affecting growth and survival of black soldier fly (BSFL), housefly (HFL), and lesser mealworm (LMW) larvae. However, the genotoxic safety of larvae reared on AFB-contaminated substrate remains uncertain. This study assessed the genotoxic effects of larvae reared on 0, 20, and 200 µg/kg AFB spiked substrate through in vitro testing. AFB and metabolites were analysed in the feed substrate, larvae and residual material using a LC-MS/MS-based method. Cytotoxic and genotoxic potential of larval extracts were assessed using the Ames MPF™ assay (mini-Ames assay), Ames test, and in vitro micronucleus assay. RESULTS: indicated no effect on survival and biomass for all insects and no bioaccumulation of AFB. Species-specific AFB metabolism appeared to occur. HFL exhibited no cytotoxic or genotoxic potential. Further research is required for BSFL and LMW, due to matrix effects and incompatibility with the used in vitro tests.
受黄曲霉毒素B（AFB）污染的作物可作为昆虫饲料，且不会影响黑水虻（BSFL）、家蝇（HFL）和黄粉虫（LMW）幼虫的生长与存活。然而，在受AFB污染的基质上饲养的幼虫的遗传毒性安全性仍不确定。本研究通过体外测试评估了在添加0、20和200µg/kg AFB的基质上饲养的幼虫的遗传毒性效应。使用基于LC-MS/MS的方法分析了饲料基质、幼虫和残留物质中的AFB及其代谢产物。使用Ames MPF™ 试验（微型Ames试验）、Ames试验和体外微核试验评估幼虫提取物的细胞毒性和遗传毒性潜力。结果表明，对所有昆虫的存活和生物量均无影响，且AFB没有生物累积现象。似乎发生了物种特异性的AFB代谢。HFL没有细胞毒性或遗传毒性潜力。由于基质效应以及与所使用的体外试验不兼容，对BSFL和LMW还需要进一步研究。
