Wang Lu-Yu, Xiao Lin, Ren Tian-Yu, Cheng Ling-Xin, Xiong Jun-Han, Fan Zheng, Zhang Zhi-Sheng
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Sciences, Southwest University, Chongqing, 400715, China.
Sci Data. 2025 May 24;12(1):859. doi: 10.1038/s41597-025-05215-1.
The marbled orb-weaver spider, Araneus marmoreus (Araneae: Araneidae), is distinguished by its unique inflated, pumpkin-like abdomen. Numerous genome studies have been conducted on Araneidae species, providing insights into their unique biological traits. However, studies on A. marmoreus remain limited, despite its ecological significance and intriguing morphology. The lack of a high-quality reference genome has further hindered in-depth exploration of its evolutionary biology and ecological dynamics. Here, we present a chromosome-level genome assembly for A. marmoreus, generated using a combination of Illumina, PacBio, and Hi-C sequencing technologies. The assembled genome is 2.39 Gb in size, comprising 13 chromosomes, with a scaffold N50 of 181.8 Mb and a contig N50 of 721.3 kb. The assembly achieved a BUSCO completeness score of 97.1% (n = 2,934), including 91.0% complete and single-copy BUSCOs and 6.1% complete and duplicated BUSCOs. Repetitive sequences accounted for 59.25% of the genome, and 23,381 protein-coding genes were annotated. This high-quality genome provides a valuable resource for advancing research into the evolutionary genomics and ecological dynamics of A. marmoreus.
大理石花纹球蛛（Araneus marmoreus，蜘蛛目：园蛛科）以其独特的膨胀的南瓜状腹部而闻名。已经对园蛛科物种进行了大量的基因组研究，为了解它们独特的生物学特性提供了见解。然而，尽管大理石花纹球蛛具有生态意义和引人入胜的形态，但对其的研究仍然有限。高质量参考基因组的缺乏进一步阻碍了对其进化生物学和生态动态的深入探索。在这里，我们展示了一个使用Illumina、PacBio和Hi-C测序技术组合生成的大理石花纹球蛛的染色体水平基因组组装。组装后的基因组大小为2.39 Gb，由13条染色体组成，支架N50为181.8 Mb，重叠群N50为721.3 kb。该组装的BUSCO完整性得分达到97.1%（n = 2,934），包括91.0%完整且单拷贝的BUSCO和6.1%完整且重复的BUSCO。重复序列占基因组的59.25%，并注释了23,381个蛋白质编码基因。这个高质量的基因组为推进对大理石花纹球蛛的进化基因组学和生态动态的研究提供了宝贵的资源。
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