Jones C A, Tsukamoto T, O'Brien P C, Uhl C B, Alley M C, Lieber M M
Br J Cancer. 1985 Sep;52(3):303-10. doi: 10.1038/bjc.1985.194.
In vitro drug sensitivity testing, both by optical colony counting and by a [3H]-TdR incorporation assay, was performed on human tumour cells proliferating in soft agar cultures. Cells from two different human tumour cell lines, 5 different human tumour xenografts, and 94 different primary human tumour specimens of various histologic types were studied. Regression analysis comparing the results of the colony counting assay and the [3H]-TdR assay revealed good to excellent correlations between the two assay endpoints for quantitating the effect of in vitro anticancer drug exposure for a large number of different agents. The presence of pre-existing tumour cell aggregates complicates the performance of the optical colony counting assay. The [3H]-TdR incorporation assay is more sensitive and reproducible than the colony counting assay when performed on samples containing a large number of initially seeded tumour cell aggregates.
采用光学集落计数法和[3H]-胸腺嘧啶核苷掺入法，对在软琼脂培养中增殖的人肿瘤细胞进行了体外药敏试验。研究了来自两种不同人肿瘤细胞系、5种不同人肿瘤异种移植物以及94种不同组织学类型的原发性人肿瘤标本的细胞。通过回归分析比较集落计数法和[3H]-胸腺嘧啶核苷掺入法的结果，发现对于大量不同药物，在定量体外抗癌药物暴露的影响时，这两种检测终点之间具有良好至极佳的相关性。预先存在的肿瘤细胞聚集体的存在使光学集落计数法的操作变得复杂。当对含有大量初始接种肿瘤细胞聚集体的样本进行检测时，[3H]-胸腺嘧啶核苷掺入法比集落计数法更灵敏且可重复。
