Tsushima K, Podratz K C, Stanhope C R, Lieber M M
Department of Urology, Mayo Clinic, Rochester, Minnesota 55905.
Gynecol Oncol. 1987 Oct;28(2):170-80. doi: 10.1016/0090-8258(87)90211-3.
Optical colony counting was used for assessing drug sensitivity of 87 samples (from 51 primary solid tumors, 19 metastatic solid tumors, and 17 ascites fluids) from 83 common epithelial type ovarian carcinomas. The evaluability rate by the optical counting method was 26.4%. Fifty of these samples were also assessed by a tritiated thymidine ( [3H]dThd) incorporation assay (evaluability rate by incorporation assay, 74%). In the 12 cases fully evaluable by both methods, the correlation coefficients for regression of drug sensitivity data between the methods ranged from 0.503 to 0.976 (mean, 0.745); for the combined data of these cases, the correlation coefficient was 0.707. The new [3H]dThd assay appears useful and efficient for performing in vitro chemotherapy sensitivity testing of human ovarian cancer.
采用光学菌落计数法评估了来自83例常见上皮型卵巢癌的87个样本（51个原发性实体瘤、19个转移性实体瘤和17个腹水样本）的药物敏感性。光学计数法的可评估率为26.4%。其中50个样本还通过氚标记胸腺嘧啶核苷（[3H]dThd）掺入试验进行了评估（掺入试验的可评估率为74%）。在两种方法均可完全评估的12例病例中，两种方法之间药物敏感性数据回归的相关系数范围为0.503至0.976（平均为0.745）；对于这些病例的合并数据，相关系数为0.707。新的[3H]dThd试验对于进行人卵巢癌的体外化疗敏感性测试似乎是有用且有效的。
