用于评估抗癌药物暴露24小时后细胞毒性的体外试验的相互比较。

An intercomparison of in vitro assays for assessing cytotoxicity after a 24 hour exposure to anti-cancer drugs.

作者信息

Rupniak H T, Dennis L Y, Hill B T

出版信息

Tumori. 1983 Feb 28;69(1):37-42. doi: 10.1177/030089168306900106.

Abstract

Employing a tumourigenic mouse cell line, we have measured the effects of four anti-cancer drugs (methotrexate, vincristine, cis-platinum and adriamycin) upon tumour cell survival as assessed by three independent procedures. The results from two short-term procedures based upon dye exclusion and labeling index determinations were compared with data from the relatively long-term clonogenic or colony-forming assay. The dye exclusion procedure demonstrated the poorest correlation with the clonogenic assay, whereas labeling index measurements exhibited qualitative but not quantitative correspondence with results from the clonogenic assay. These short-term procedures cannot therefore be considered accurate substitutes for a clonogenic assay, which remains the method of choice for assessing cytotoxic effects of anti-cancer drugs.

摘要

利用一种致瘤小鼠细胞系，我们通过三种独立的方法测量了四种抗癌药物（甲氨蝶呤、长春新碱、顺铂和阿霉素）对肿瘤细胞存活的影响。将基于染料排斥和标记指数测定的两种短期方法的结果与相对长期的克隆形成或集落形成试验的数据进行了比较。染料排斥法与克隆形成试验的相关性最差，而标记指数测量与克隆形成试验的结果在定性上相符，但在定量上不相符。因此，这些短期方法不能被认为是克隆形成试验的准确替代方法，克隆形成试验仍然是评估抗癌药物细胞毒性作用的首选方法。