Clausen H, Levery S B, McKibbin J M, Hakomori S
Biochemistry. 1985 Jul 2;24(14):3578-86. doi: 10.1021/bi00335a028.
Application of a monoclonal antibody defining monofucosyl type 1 chain A (AH21) revealed the presence of a glycolipid having the same thin-layer chromatography mobility as Aa but showing a clear reactivity with AH21. This glycolipid was detectable in Lea-b- erythrocytes but not in Lea+b- or Lea-b+ erythrocytes. Another monoclonal antibody defining difucosyl type 1 chain A (HH3) detected the presence of a glycolipid component reacting with this antibody in Lea-b+ erythrocytes but not in Lea+b- or Lea-b- erythrocytes. The component defined by monoclonal antibody AH21 and that defined by HH3 were isolated and characterized by 1H NMR spectrometry and methylation analysis as having the structures (Formula: see text) The 1H NMR spectra of these glycolipid antigens were characterized by resonances for anomeric protons that are identical with those of glycolipids with type 1 chain previously isolated but distinctively different from those of type 2 chain analogues. Resonances reflecting ceramide composition are characteristic for these antigens from human erythrocytes and are distinguishable from those of the same antigen from other sources.
一种定义单岩藻糖基1型A链（AH21）的单克隆抗体的应用表明，存在一种糖脂，其在薄层色谱中的迁移率与Aa相同，但与AH21有明显反应。这种糖脂在Lea-b-红细胞中可检测到，但在Lea+b-或Lea-b+红细胞中未检测到。另一种定义二岩藻糖基1型A链（HH3）的单克隆抗体在Lea-b+红细胞中检测到一种与该抗体反应的糖脂成分，但在Lea+b-或Lea-b-红细胞中未检测到。通过1H NMR光谱和甲基化分析对单克隆抗体AH21定义的成分和HH3定义的成分进行了分离和表征，其结构为（化学式：见正文）。这些糖脂抗原的1H NMR光谱的特征在于异头质子的共振，其与先前分离的具有1型链的糖脂的异头质子共振相同，但与2型链类似物的异头质子共振明显不同。反映神经酰胺组成的共振对于来自人红细胞的这些抗原具有特征性，并且与来自其他来源的相同抗原的共振可区分。
