Kannagi R, Roelcke D, Peterson K A, Okada Y, Levery S B, Hakomori S
Carbohydr Res. 1983 Aug 16;120:143-57. doi: 10.1016/0008-6215(83)88013-6.
The antibody Fl shows preferential reactivity with adult erythrocytes over newborn erythrocytes, and its reactivity is abolished by sialidase treatment of the erythrocyte. The antibody was found to recognize binary determinants linked to the branched lacto-N-isooctaosylceramide (formula; see text) The presence of an N-acetylneuraminyl group at one end and L-fucosyl group at the other end is essential for the reactivity of the antibody. A substitution at the penultimate D-galactosyl residue of one of the chains with an alpha-D-(1 leads to 3)-linked 2-acetamido-2-deoxygalactosyl or galactosyl group did not inhibit the reactivity of the antibody. The new blood group A- and B-active, branched gangliosides are also isolated and characterized.
抗体Fl对成年红细胞的反应性优于新生红细胞，并且通过对红细胞进行唾液酸酶处理可消除其反应性。发现该抗体识别与分支乳糖-N-异辛糖神经酰胺（分子式；见正文）相关的二元决定簇。一端存在N-乙酰神经氨酸基团而另一端存在L-岩藻糖基团对于该抗体的反应性至关重要。一条链的倒数第二个D-半乳糖残基被α-D-(1→3)-连接的2-乙酰氨基-2-脱氧半乳糖基或半乳糖基取代不会抑制该抗体的反应性。新的具有A和B血型活性的分支神经节苷脂也被分离和鉴定。
