Pliashkevich Iu G, Demushkin V P
Biokhimiia. 1985 Jul;50(7):1167-74.
The effect of chemical modification on the binding of cholinergic ligands to nicotinic acetylcholine receptor from squid optical ganglion was studied. The existence of two chemically distinct subpopulations of binding sites was postulated. Subpopulation I contains, in all probability, Arg, Tyr and carboxyl groups critical for the binding of both ligands. Subpopulation II differs from the first one in the amino group present instead of Arg. The amino group important for the binding of d-tubocurarine alone was found in both subpopulations. The data obtained allow one to construct a model of nicotinic acetylcholine receptor cholinergic ligand recognition sites.
研究了化学修饰对鱿鱼视神经节烟碱型乙酰胆碱受体与胆碱能配体结合的影响。推测存在两个化学性质不同的结合位点亚群。亚群I很可能含有对两种配体结合至关重要的精氨酸、酪氨酸和羧基。亚群II与第一个亚群的不同之处在于存在氨基而非精氨酸。在两个亚群中都发现了仅对d -筒箭毒碱结合重要的氨基。所获得的数据使人们能够构建烟碱型乙酰胆碱受体胆碱能配体识别位点的模型。
