Kossalbayev Bekzhan D, Wei Mo, Wang Jingjing, Pang Yan, Lv Mingda, Sadvakasova Asemgul K, Bauenova Meruyert O, Zhang Xiaoxia, Zhao Wei, Xu Song, Huang Zhiyong
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, 050038, Kazakhstan.
World J Microbiol Biotechnol. 2025 May 26;41(6):181. doi: 10.1007/s11274-025-04403-w.
The construction of efficient synthetic microbial communities (SMCs) is a major challenge for the scientific community. In this study, we constructed 15 SMCs with a diversity gradient of 1-4 by combining four strains with different functions, and investigated the effects of strain interactions, community diversity and in vitro functions on in vivo rice growth promotion in two soil types. The interactions between the 4 strains were tested using a modified dual culture plate assay. The in vitro functions (nitrogen fixation, phosphate and potassium solubilization, indoleacetic acid production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, siderophore production) of the 15 SMCs were measured by the biochemical assay. The in vivo growth promotion of the 15 SMCs was investigated in rice pot experiments in 2 different soils. The study showed that the in vivo functions of SMCs were related to the soils in which they were grown and their in vitro functions and diversity. The in vitro functions of SMCs were closely related to the functions (especially ACC deaminase producing capabilities), diversity, and interactions of the constituent strains. It was concluded that the plant growth promotion of SMCs was influenced by the function, diversity and interactions of their constituent strains and soil types. This not only advances our understanding of microbe-microbe-plant interactions, but also sheds light on the rational design of effective SMCs for environmentally friendly agriculture.
构建高效的合成微生物群落（SMC）是科学界面临的一项重大挑战。在本研究中，我们通过组合四种具有不同功能的菌株构建了15个多样性梯度为1至4的SMC，并研究了菌株相互作用、群落多样性和体外功能对两种土壤类型中水稻体内生长促进作用的影响。使用改良的双培养平板试验测试了这4种菌株之间的相互作用。通过生化分析测定了15个SMC的体外功能（固氮、溶解磷酸盐和钾、产生吲哚乙酸、1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性、产生铁载体）。在两种不同土壤的水稻盆栽试验中研究了15个SMC的体内生长促进作用。研究表明，SMC的体内功能与其生长的土壤以及体外功能和多样性有关。SMC的体外功能与组成菌株的功能（特别是产生ACC脱氨酶的能力）、多样性和相互作用密切相关。得出的结论是，SMC对植物生长的促进作用受其组成菌株的功能、多样性和相互作用以及土壤类型的影响。这不仅增进了我们对微生物-微生物-植物相互作用的理解，也为合理设计用于环境友好型农业的有效SMC提供了思路。
