Keefer C L, Bennett K A, Brackett B G
Biol Reprod. 1985 Sep;33(2):388-92. doi: 10.1095/biolreprod33.2.388.
Rabbit ovum donors were superovulated with pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Ova were recovered 16-17 h post-hCG from oviducts immediately after killing and from excised oviducts held in saline 30 min at 33 degrees or 38 degrees C prior to ovum recovery. In vivo-capacitated spermatozoa were used to inseminate both groups of ova. Data revealed a decrease in fertilization rates following a 30-min delay at 38 degrees C in ovum recovery. Thus, 64% (44/69 ova) were fertilized with rapid recovery, whereas 43% (39/90 ova) were fertilized following a 30-min delay. The decrease in fertilization imposed by delay in ovum recovery was apparently overcome when oviduct storage was at 33 degrees C. Under these conditions, 69% of inseminated ova were fertilized. Ova inseminated with in vitro-capacitated sperm showed a similar response to delayed ovum recovery. Embryonic development in culture of ova obtained from mated does was not affected by delay in recovery at 33 degrees or 38 degrees C provided mated does had been injected only with hCG. Ova from mated does receiving both PMSG and hCG were adversely affected by a 38 degrees C delay. The data emphasize the importance of rapid ovum recovery from oviducts and suggest the possibility of altering conditions to overcome damaging effects of delayed recovery.
兔卵供体用孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）进行超排卵处理。在注射hCG后16 - 17小时，处死兔子后立即从输卵管中回收卵子，以及在回收卵子前30分钟将切除的输卵管置于33℃或38℃的盐水中后回收卵子。用体内获能的精子对两组卵子进行授精。数据显示，在38℃下延迟30分钟回收卵子后受精率下降。因此，快速回收时64%（44/69个卵子）受精，而延迟30分钟后43%（39/90个卵子）受精。当输卵管在33℃保存时，卵子回收延迟所造成的受精率下降显然被克服。在这些条件下，69%的授精卵子受精。用体外获能精子授精的卵子对延迟回收卵子表现出类似的反应。如果交配母兔仅注射hCG，则在33℃或38℃下回收延迟对从交配母兔获得的卵子在培养中的胚胎发育没有影响。接受PMSG和hCG的交配母兔的卵子在38℃延迟回收时受到不利影响。这些数据强调了从输卵管快速回收卵子的重要性，并表明有可能改变条件以克服延迟回收的有害影响。
