Brackett B G, Hall J L, Oh Y K
Fertil Steril. 1978 May;29(5):571-82.
The fertilizing ability of testicular, epididymal, and ejaculated rabbit spermatozoa was evaluated in vitro following in vitro capacitation by high ionic strength treatment. Fewer than 11% of inseminated ova were apparently fertilized (i.e., in pronuclear, two-, and four-cell stages as determined by light microscopy) when testicular sperm treated with caffeine, caput epididymal, or corpus epididymal sperm samples were tested. A greater fertilizing ability, reflected by the percentage of ova fertilized and more normal progression of embryonic development, was exhibited by cauda epididymal sperm. Of 93 ova, 68 (73.1%) were fertilized by cauda sperm, whereas ejaculated sperm from the same 10 bucks fertilized 34 (36.6%) of 93 ova (P is less than 0.005). Ultrastructural examination of selected ova apparently fertilized by sperm from levels of the male reproductive tract proximal to the cauda epididymidis revealed abnormal activation. Authentic fertilization occurred when ova were inseminated with cauda epididymal and ejaculated sperm. An unusual and infrequent form of activation involving failure of cortical granule breakdown in ova penetrated by cauda epididymal and ejaculated sperm was seen. A comparison of fertilizing ability of sperm from first, second, and third ejaculates revealed a significant decrease with the third ejaculate (P is less than 0.01).
通过高离子强度处理进行体外获能后，对兔睾丸、附睾及射出精子的受精能力进行了体外评估。当测试用咖啡因处理的睾丸精子、附睾头或附睾体精子样本时，明显受精的受精卵（即通过光学显微镜确定处于原核、二细胞和四细胞阶段）少于11%。附睾尾精子表现出更强的受精能力，表现为受精卵的百分比更高以及胚胎发育进程更正常。93枚卵子中，68枚（73.1%）被附睾尾精子受精，而来自同一10只雄兔的射出精子使93枚卵子中的34枚（36.6%）受精（P<0.005）。对明显被附睾尾近端雄性生殖道水平的精子受精的选定卵子进行超微结构检查，发现激活异常。当用附睾尾和射出精子对卵子进行授精时，发生了真正的受精。观察到一种不寻常且罕见的激活形式，即被附睾尾和射出精子穿透的卵子中皮质颗粒分解失败。对首次、第二次和第三次射精的精子受精能力进行比较，发现第三次射精时受精能力显著下降（P<0.01）。
