Ouyang Xin, Chen Senhua, Chen Qiling, Guo Heng, Liu Lan, Liu Hongju, Yan Chong
School of Pharmacy, Guangdong Medical University, Dongguan, China.
School of Marine Sciences, Sun Yat-sen University, Zhuhai, China.
Mycology. 2024 Oct 16;16(2):918-928. doi: 10.1080/21501203.2024.2397600. eCollection 2025.
Four new lactones, including hypomonacid A () and hypomonone A-C (), as well as nine known polyketide analogues ( and ) were obtained from endophytic fungus sp. TX-09 derived from . Their planar structures were extensively established by analysing HRESIMS and NMR spectroscopic data. Stereochemistry of new compounds was determined by X-ray diffraction analysis and modified Mosher's method in combination with quantum-chemical ECD calculation. In addition, compounds and showed anti-inflammatory activity by inhibition of lipopolysaccharide (LPS)-induced NO production in RAW264.7 cells at 50 μmol/L without cytotoxicity. Among them, compound inhibited the production of LPS-stimulated inflammation in mouse macrophage RAW264.7 cells by suppressing the expression of iNOS, TNF-α, IL-1β, and IL-6.
从来源于[具体来源未给出]的内生真菌[真菌名称未给出]TX-09中获得了四种新的内酯，包括次单酸A（[具体结构未给出]）和次单酮A - C（[具体结构未给出]），以及九种已知的聚酮类似物（[具体结构未给出]）。通过分析高分辨电喷雾电离质谱（HRESIMS）和核磁共振（NMR）光谱数据广泛确定了它们的平面结构。新化合物的立体化学通过X射线衍射分析以及改良的莫舍尔方法结合量子化学电子圆二色（ECD）计算来确定。此外，化合物[具体化合物未给出]和[具体化合物未给出]在50μmol/L时通过抑制脂多糖（LPS）诱导的RAW264.7细胞中一氧化氮（NO）的产生显示出抗炎活性且无细胞毒性。其中，化合物[具体化合物未给出]通过抑制诱导型一氧化氮合酶（iNOS）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）的表达，抑制了LPS刺激的小鼠巨噬细胞RAW264.7细胞中的炎症产生。
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