He Yan, Wang Jun, Li Junjiao, Wang Xiayu, Yang Xingyong, Chen Jieyin, Zhang Dandan
College of Pharmacy, Chengdu University, Chengdu, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Mycology. 2024 Nov 8;16(2):876-890. doi: 10.1080/21501203.2024.2419882. eCollection 2025.
CAP (cysteine-rich secretory proteins, antigen5, pathogenesis-related proteins) superfamily proteins are widely distributed, can be subdivided into 11 subfamilies, and form a unique branch in fungi, named PRY proteins. is a soil-borne fungal pathogen of vascular plants that causes plant Verticillium wilt. However, the roles of CAP superfamily proteins in this fungus is unclear. Here, four CAP superfamily members with a conserved domain were identified in : VdPRY1, VdPRY2, VdPRY3, and VdPRY4. VdPRY1 and VdPRY3 were found to be key in suppressing plant immune responses. Moreover, these four members are highly expressed during early infection of cotton by . Deleting , , or reduced the fungus's ability to cause disease, but deletion did not affect virulence. Deletion of any of four members did not impact fungal growth or carbon source use. Yeast two-hybrid experiments suggest that these proteins may function through interactions with each other. This investigation has, for the initial time, elucidated the pivotal roles of CAP superfamily proteins in inhibiting plant immunity and exerting virulence during interaction with the host plant.
富含半胱氨酸的分泌蛋白、抗原5、病程相关蛋白（CAP）超家族蛋白广泛分布，可细分为11个亚家族，在真菌中形成一个独特的分支，称为PRY蛋白。黄萎病菌是一种引起植物黄萎病的土传维管束植物真菌病原体。然而，CAP超家族蛋白在这种真菌中的作用尚不清楚。在此，在黄萎病菌中鉴定出四个具有保守结构域的CAP超家族成员：VdPRY1、VdPRY2、VdPRY3和VdPRY4。发现VdPRY1和VdPRY3在抑制植物免疫反应中起关键作用。此外，这四个成员在黄萎病菌早期侵染棉花期间高表达。缺失VdPRY1、VdPRY2或VdPRY3会降低真菌的致病能力，但缺失VdPRY4不影响毒力。缺失这四个成员中的任何一个都不会影响真菌生长或碳源利用。酵母双杂交实验表明，这些蛋白可能通过相互作用发挥功能。本研究首次阐明了黄萎病菌CAP超家族蛋白在与寄主植物相互作用过程中抑制植物免疫和发挥毒力方面的关键作用。
