Zhang Yu, Dhanasekaran Solairaj, Ngea Guillaume Legrand Ngolong, Yang Qiya, Zhang Hongyin
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
Food Chem. 2023 Aug 15;417:135785. doi: 10.1016/j.foodchem.2023.135785. Epub 2023 Feb 26.
The intracellular enzymes of antagonistic yeast are effective in controlling patulin (PAT) contamination. However, countless enzymes that have been revealed remain functionally uncharacterized. The study built on previous transcriptomic data obtained by our research group to amplify and express a gene encoding a short-chain dehydrogenase/reductase (SDR) in Meyerozyma guilliermondii. Overexpression of SDR increased the tolerance of M. guilliermondii to PAT and the ability to degrade PAT of the intracellular enzymes. Furthermore, MgSDR-overexpressed M. guilliermondii showed higher PAT degradation in juices (apple and peach) and controlled the blue mold of pears at 20 °C and 4 °C while significantly reduced the content of PAT and the biomass of Penicillium expansum in decayed tissues than wild-type M. guilliermondii. This study provides theoretical references for the subsequent heterologous expression, formulation, and application of the SDR protein from M. guilliermondii and contributes to elucidating the PAT degradation mechanism of antagonistic yeasts.
拮抗菌酵母的细胞内酶在控制展青霉素（PAT）污染方面有效。然而，已发现的无数种酶在功能上仍未得到表征。本研究基于我们研究小组先前获得的转录组数据，在季也蒙毕赤酵母中扩增并表达了一个编码短链脱氢酶/还原酶（SDR）的基因。SDR的过表达提高了季也蒙毕赤酵母对PAT的耐受性以及细胞内酶降解PAT的能力。此外，过表达MgSDR的季也蒙毕赤酵母在果汁（苹果汁和桃汁）中表现出更高的PAT降解能力，并且在20℃和4℃下能控制梨的青霉病，与野生型季也蒙毕赤酵母相比，其在腐烂组织中显著降低了PAT含量和扩展青霉的生物量。本研究为后续季也蒙毕赤酵母SDR蛋白的异源表达、制剂开发和应用提供了理论参考，并有助于阐明拮抗菌酵母的PAT降解机制。
