Eremin Andrey, Sergeev Alexander, Kopylov Arthur, Rodin Vladimir, Malyshev Daniil, Panova Tatiana, Polyakov Igor, Zvereva Maria
Lomonosov Moscow State University, Moscow, Russia.
Institute of Biomedical Chemistry, Moscow, Russia.
Front Genet. 2025 May 9;16:1574332. doi: 10.3389/fgene.2025.1574332. eCollection 2025.
is a versatile thermotolerant organism with numerous applications in biotechnology, particularly in the production of recombinant proteins and the study of methanol metabolism and peroxisome functions. This study presents a comprehensive genome and methylome analysis of using long-read sequencing technology. The research builds upon previous short-read sequencing efforts, revealing enhancements in genome assembly and epigenomic insights.
We used long-read sequencing technology to achieve a telomere-to-telomere (T2T) genome assembly of . High-quality reads were obtained and assembled , followed by polishing to enhance accuracy. The genome was analyzed to identify coding genes, telomeric motifs, rRNA genes, and methylation patterns, including the detection of 5mC and 6 mA modifications. Epigenetic features were further assessed and validated through liquid chromatography-mass spectrometry.
Key findings include the absence of 5 mC DNA modification and the presence of 6 mA in the genome, unusual telomere regulation mechanism based on the addition of non-telomeric dT and the introduction of long-read enhanced telomere-to-telomere assembly.
This work provides deeper insights into the yeast's genome organization and methylation patterns, contributing to the understanding of its genetics and therefore potential biotechnological applications.
是一种多功能耐热生物，在生物技术领域有众多应用，尤其在重组蛋白生产以及甲醇代谢和过氧化物酶体功能研究方面。本研究利用长读长测序技术对进行了全面的基因组和甲基化组分析。该研究基于先前的短读长测序工作，揭示了基因组组装和表观基因组学见解方面的改进。
我们使用长读长测序技术实现了的端粒到端粒（T2T）基因组组装。获得高质量 reads 并进行组装，随后进行抛光以提高准确性。对基因组进行分析以识别编码基因、端粒基序、rRNA 基因和甲基化模式，包括检测 5mC 和 6mA 修饰。通过液相色谱 - 质谱进一步评估和验证表观遗传特征。
主要发现包括基因组中不存在 5mC DNA 修饰但存在 6mA，基于非端粒 dT 添加的异常端粒调控机制以及长读长增强的端粒到端粒组装的引入。
这项工作为酵母的基因组组织和甲基化模式提供了更深入的见解，有助于理解其遗传学以及潜在的生物技术应用。
