Liu Kangjing, Tian Xueying, Fei Siyuan, Song Yukun, Abd El-Aty A M, Tan Mingqian
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian, 116034 China.
School of Food Science and Technology, Academy of Food Interdisciplinary Science, Dalian Polytechnic University, Dalian, 116034 China.
Mar Life Sci Technol. 2025 Feb 17;7(2):352-365. doi: 10.1007/s42995-024-00255-9. eCollection 2025 May.
This study demonstrated the design of whey protein isolate (WPI)-mannose (Man) conjugates with triphenylphosphonium bromide (TPP) through self-assembly to prepare macrophage and mitochondrion dual-targeting astaxanthin (AXT) nanoparticles (AXT@TPP-WPI-Man). The nanoparticles displayed spherical structures with a well-dispersed size of approximately 206.1 ± 39.2 nm, with good biocompatibility, stability, and targeting capabilities. In vitro experiments demonstrated the specific accumulation of AXT@TPP-WPI-Man in mitochondria and exhibited good targeting ability toward macrophages. The AXT@TPP-WPI-Man effectively reduced reactive oxygen species and preserved the normal mitochondrial membrane potential. The AXT@TPP-WPI-Man treated ulcerative colitis mice exhibited a 52.32% increase in colon length with significant improvement in weight loss, disease activity index scores, and reduced release of inflammatory cytokines. Immunofluorescence staining indicated AXT@TPP-WPI-Man alleviated ulcerative colitis by reducing M1 polarization in colonic macrophages while promoting M2 polarization. The dual-targeting AXT@TPP-WPI-Man has the potential to improve astaxanthin bioavailability, presenting a promising delivery method for the treatment of ulcerative colitis.
The online version contains supplementary material available at 10.1007/s42995-024-00255-9.
本研究展示了通过自组装设计乳清分离蛋白（WPI）-甘露糖（Man）与溴化三苯基鏻（TPP）的缀合物，以制备巨噬细胞和线粒体双靶向虾青素（AXT）纳米颗粒（AXT@TPP-WPI-Man）。这些纳米颗粒呈现球形结构，尺寸约为206.1±39.2纳米，分散良好，具有良好的生物相容性、稳定性和靶向能力。体外实验表明AXT@TPP-WPI-Man在线粒体中特异性积累，并对巨噬细胞表现出良好的靶向能力。AXT@TPP-WPI-Man有效减少活性氧并维持正常的线粒体膜电位。经AXT@TPP-WPI-Man处理的溃疡性结肠炎小鼠结肠长度增加了52.32%，体重减轻、疾病活动指数评分显著改善，炎症细胞因子释放减少。免疫荧光染色表明AXT@TPP-WPI-Man通过减少结肠巨噬细胞中的M1极化同时促进M2极化来减轻溃疡性结肠炎。双靶向的AXT@TPP-WPI-Man有潜力提高虾青素的生物利用度，为溃疡性结肠炎的治疗提供了一种有前景的递送方法。
在线版本包含可在10.1007/s42995-024-00255-9获取的补充材料。
