Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, Liaoning, P. R. China.
Food Funct. 2021 Sep 7;12(17):7718-7727. doi: 10.1039/d1fo01751a. Epub 2021 Jul 21.
Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery. The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX were 135.26 and 193.64 nm, respectively, which exhibited a spherical structure and good dispersibility. The mitochondria targeting nanocarriers had good stability in the stimulated blood fluid. In vitro experiments indicated that the TPP-modified nanocarriers could effectively realize lysosomes escape, and specifically accumulate in the cell mitochondria. Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells. The present study highlights the promising application of mitochondria targeting nanocarriers for enhanced delivery of astaxanthin.
采用三苯基膦溴化盐（TPP）修饰的乳清蛋白分离物（WPI）-葡聚糖（DX）缀合物通过自组装法制备了新型靶向线粒体的纳米载体，用于虾青素靶向递送至线粒体。负载虾青素的 WPI-DX 和负载虾青素的 TPP-WPI-DX 的纳米载体分别为 135.26 和 193.64nm，呈现球形结构且分散性良好。刺激血液流体中的靶向线粒体纳米载体具有良好的稳定性。体外实验表明，TPP 修饰的纳米载体可以有效实现溶酶体逃逸，并特异性地聚集在线粒体中。同时，负载虾青素的纳米载体可以显著减少由过氧化氢产生的活性氧的生成，保护线粒体膜电位的正常水平，并显著促进小鼠巨噬细胞（RAW 264.7）细胞中白血病细胞的活力。本研究突出了靶向线粒体的纳米载体在增强虾青素传递方面的应用前景。
