Soiefer A I, Miller M S, Sabri M I, Spencer P S
Brain Res. 1985 Sep 2;342(1):196-9. doi: 10.1016/0006-8993(85)91375-7.
A rapid technique for separating and quantitating the three enolase isozymes present in rodent brain and sciatic nerve was developed using high-pressure liquid anion-exchange chromatography. At pH 7.9, one cationic and two anionic enzyme forms were separated with baseline resolution in an imidazole buffer containing ethylenediaminetetraacetic acid (EDTA) and magnesium. The recovery of enolase activity was 90% or greater for brain and 85% for sciatic nerve. Chromatography of liver and axon-free (degenerated) sciatic nerve allowed the identification of non-neuronal, hybrid, and neuron-specific enolase isozymes. These enzyme forms, respectively, constituted 40%, 29% and 19% of total activity in brain, and 63%, 13% and 4% of total activity in normal sciatic nerve.
采用高压液相阴离子交换色谱法，开发了一种快速分离和定量啮齿动物脑和坐骨神经中三种烯醇化酶同工酶的技术。在pH 7.9条件下，在含有乙二胺四乙酸（EDTA）和镁的咪唑缓冲液中，一种阳离子型和两种阴离子型酶形式以基线分辨率分离。脑烯醇化酶活性回收率达90%或更高，坐骨神经为85%。对肝脏和无轴突（变性）坐骨神经进行色谱分析，可鉴定出非神经元、杂交和神经元特异性烯醇化酶同工酶。这些酶形式分别占脑总活性的40%、29%和19%，占正常坐骨神经总活性的63%、13%和4%。
