Gülsu Su Mercan, Kökenek Ünal Tuba Dilay, Yılmaz Şükranlı Zeynep, Mehmetbeyoğlu Duman Ecmel, Yora Samed, Memiş Mehmet, Taheri Serpil
Betul Ziya Eren Genome and Stem Cell Center, Erciyes University, Kayseri, Turkey.
Department of Pathology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey.
Mol Biol Rep. 2025 May 26;52(1):505. doi: 10.1007/s11033-025-10599-9.
Mild traumatic brain injury (mTBI) is a significant health issue affecting people of all ages and socioeconomic groups. Its clinical spectrum alters from transient mild symptoms to disabling problems. The molecular mechanisms that affect the vital functions and treatment after mTBI have yet to be fully understood. This study aims to investigate the expression levels of Alkaline Phosphatase (Alpl) and Fibroblast Growth Factor (bFGF) in peripheral organs after mTBI in traumatic mice models and observe the differences between acute and chronic phases.
A total of 30 animals were randomly divided into three groups, with equal numbers of males and females in each group. mTBI was induced in mice utilizing the Marmarau trauma model. Alpl and bFGF expression levels in the acute and chronic phases were determined via Real-Time PCR in liver, kidney, bone, and muscle tissues. Alpl and bFGF gene expressions in the acute phase after mTBI increased significantly. In the kidney, Alpl and bFGF levels increased in the acute phase in females, but bFGF significantly decreased in males. In muscle tissue bFGF levels significantly increased in males in the chronic phase. Our study showed significant differences between sexes in response to mTBI.
Our study investigated the role of Alpl and bFGF genes in peripheral tissues in acute and chronic phases after mTBI for the first time in the literature. The data obtained will guide understanding the secondary events and the consequences of the disease in mTBI and taking the necessary treatment and measures.
轻度创伤性脑损伤（mTBI）是一个严重的健康问题，影响着所有年龄段和社会经济群体的人群。其临床症状范围从短暂的轻微症状到致残性问题不等。mTBI后影响重要功能和治疗的分子机制尚未完全明确。本研究旨在探讨创伤性小鼠模型中mTBI后外周器官中碱性磷酸酶（Alpl）和成纤维细胞生长因子（bFGF）的表达水平，并观察急性期和慢性期之间的差异。
总共30只动物被随机分为三组，每组雌雄数量相等。利用Marmarau创伤模型诱导小鼠发生mTBI。通过实时定量PCR测定肝脏、肾脏、骨骼和肌肉组织在急性期和慢性期的Alpl和bFGF表达水平。mTBI后急性期Alpl和bFGF基因表达显著增加。在肾脏中，急性期雌性Alpl和bFGF水平升高，而雄性bFGF显著降低。在肌肉组织中，慢性期雄性bFGF水平显著升高。我们的研究表明，在对mTBI的反应中存在性别差异。
我们的研究首次在文献中探讨了mTBI后急性期和慢性期外周组织中Alpl和bFGF基因的作用。所获得的数据将有助于理解mTBI中的继发性事件和疾病后果，并采取必要的治疗和措施。
