Zhang Mingya, Ren Quan, Wu Xiuqing, Liu Meng, Zhang Lin, Yuan Qiong, Lu Ning, Cao Yi
Guizhou Academy of Tobacco Science, Guiyang, 550081, Guizhou, China.
College of Tobacco, Guizhou University, Guiyang, 550025, Guizhou, China.
Antonie Van Leeuwenhoek. 2025 May 26;118(6):83. doi: 10.1007/s10482-025-02092-x.
Tobacco black shank is a significant disease that poses a severe threat to tobacco production. To identify effective biocontrol agents for combating tobacco black shank disease, a screening of 39 putative Pseudomonas strains was conducted utilizing the plate confrontation assay. Four strains exhibiting inhibitory effects were selected and further evaluated for their disease control potential in pot trials. The effects of the strains on the growth of tobacco seedlings were detected using the root-irrigation method. The results demonstrated that four Pseudomonas strains exhibited significant inhibitory effects against tobacco black shank disease. The inhibition rates of strains YC2140, YC2090, YC2202, and Tr157 against P. nicotianae were determined to be 77.20%, 73.43%, 73.07%, and 32.46%, respectively. Pot trials showed that the biocontrol efficiency of Tr157, YC2090, YC2140, YC2202 against tobacco black shank disease was 96.30%, 66.67%, 62.96%, 62.96%, respectively. In addition, the strain Tr157 was found to be capable of producing indole-3-acetic acid (IAA). Meanwhile, Tr157, YC2140, and YC2202 demonstrated the ability to solubilize phosphorus, while all the four strains were capable of solubilizing potassium and producing siderophores. These strains also promoted tobacco growth increasing the total fresh weight by 146.33%, 153.21%, 87.16% and 239.91%, respectively. Overall, this study underscores the potential of the four Pseudomonas strains as effective biocontrol agents against tobacco black shank disease, offering an eco-friendly and sustainable alternative to chemical fungicides in agriculture.
烟草黑胫病是一种对烟草生产构成严重威胁的重要病害。为了鉴定对抗烟草黑胫病的有效生物防治剂，利用平板对峙试验对39株假定的假单胞菌菌株进行了筛选。选择了4株具有抑制作用的菌株，并在盆栽试验中进一步评估它们的病害防治潜力。采用灌根法检测这些菌株对烟草幼苗生长的影响。结果表明，4株假单胞菌菌株对烟草黑胫病均表现出显著的抑制作用。菌株YC2140、YC2090、YC2202和Tr157对烟草疫霉的抑制率分别为77.20%、73.43%、73.07%和32.46%。盆栽试验表明，Tr157、YC2090、YC2140、YC2202对烟草黑胫病的生物防治效率分别为96.30%、66.67%、62.96%、62.96%。此外，发现菌株Tr157能够产生吲哚-3-乙酸（IAA）。同时，Tr157、YC2140和YC2202表现出溶解磷的能力，而所有4株菌株都能够溶解钾并产生铁载体。这些菌株还促进了烟草生长，使总鲜重分别增加了146.33%、153.21%、87.16%和239.91%。总体而言，本研究强调了这4株假单胞菌菌株作为对抗烟草黑胫病的有效生物防治剂的潜力，为农业中化学杀菌剂提供了一种生态友好且可持续的替代方案。
