Walz W, Hinks E C
Brain Res. 1985 Sep 16;343(1):44-51. doi: 10.1016/0006-8993(85)91156-4.
Potassium accumulation and water transport into mouse astrocytes in primary cultures were investigated when external potassium was increased from 3 to 12 mM. The intracellular potassium content increased by 63% within 50 s of such a change. The increase consisted of a ouabain- and furosemide-sensitive component, both contributing in about the same amounts. Experiments with altered ion composition revealed that the furosemide-sensitive component consisted of a KCl accumulation. Water moved into the astrocytes without delay after such an external K+ increase and increased the cell water by 27%. This water increase was abolished in solutions with reduced Cl- and during application of furosemide. Thus, these results on a KCl uptake accompanied by water movements into astrocytes suggest a potential mechanism by which glial cells in situ can regulate external K+ levels.
当原代培养的小鼠星形胶质细胞外钾浓度从3 mM增加到12 mM时，研究了钾的积累和水向细胞内的转运情况。这种变化发生后的50秒内，细胞内钾含量增加了63%。增加的部分包括哇巴因和呋塞米敏感成分，二者贡献量大致相同。离子组成改变的实验表明，呋塞米敏感成分由氯化钾的积累组成。细胞外钾离子增加后，水立即进入星形胶质细胞，细胞内水量增加了27%。在氯离子浓度降低的溶液中以及应用呋塞米期间，这种水量增加被消除。因此，这些关于氯化钾摄取伴随水进入星形胶质细胞的结果提示了一种原位神经胶质细胞调节细胞外钾离子水平的潜在机制。
