Ranner Josef L, Stabl Georg, Martyniak Cindy, Paries Michael, Spaccasassi Andrea, Gutjahr Caroline, Stark Timo D, Dawid Corinna
Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich (TUM), Lise-Meitner-Str. 34, Freising 85354, Germany.
Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Golm, Potsdam 14476, Germany.
J Agric Food Chem. 2025 Jun 4;73(22):14044-14057. doi: 10.1021/acs.jafc.5c02061. Epub 2025 May 26.
In the present study, a highly specific, accurate, and robust ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous quantification of 50 plant (poly)phenol analytes was developed and validated to assess the effect of arbuscular mycorrhizal (AM) symbiosis on the (poly)phenolic content of the model legume (). Determination of molar concentrations of analytes in roots and shoots of wild-type and AM mutant (with and without AM symbiosis, respectively) revealed an overall increase in (poly)phenols in mycorrhizal plants. Time-course observation over 10 weeks showed a shift in (poly)phenol concentrations, especially in the roots. In total, 13 analytes were notably more abundant in young AM roots, suggesting a potential role in symbiosis initiation. An accumulation of various (poly)phenols at later stages of symbiosis might indicate a potential involvement in arbuscule degradation or AM autoregulation.
在本研究中，开发并验证了一种高特异性、准确且稳健的超高效液相色谱-串联质谱法（UHPLC-MS/MS），用于同时定量50种植物（多）酚类分析物，以评估丛枝菌根（AM）共生对模式豆科植物（）（多）酚含量的影响。对野生型和AM突变体（分别具有和不具有AM共生）的根和地上部分析物摩尔浓度的测定表明，菌根植物中（多）酚类物质总体增加。为期10周的时间进程观察显示（多）酚浓度发生了变化，尤其是在根中。总共13种分析物在年轻的AM根中含量显著更高，表明其在共生起始中可能发挥作用。共生后期各种（多）酚类物质的积累可能表明其潜在参与丛枝降解或AM自调节。
