Brenowitz E A, Arnold A P
Brain Res. 1985 Sep 30;344(1):172-5. doi: 10.1016/0006-8993(85)91205-3.
Tritiated testosterone was injected into bay wrens (Thryothorus nigricapillus), a neotropical species in which the female sings a complex song in intricately timed vocal duets with males. Autoradiographic analysis indicated that male and female wrens have the same proportion of cells labeled by testosterone or its metabolites in two brain regions involved in song: the caudal nucleus of the ventral hyperstriatum (HVc) and the magnocellular nucleus of the anterior neostriatum (MAN). This contrasts with the zebra finch, a species in which only the males sing: a considerably greater proportion of male zebra finch cells in HVc and MAN are labeled than in females. This suggests that female birds that produce complex vocalizations have evolved neural song control systems that are extremely similar to those of males in steroid hormone sensitivity.
将氚标记的睾酮注射到海湾鹪鹩（黑顶鸫鹩）体内，这是一种新热带物种，其雌性会在与雄性复杂的定时声乐二重唱中唱出复杂的歌声。放射自显影分析表明，雄性和雌性鹪鹩在与鸣叫相关的两个脑区中，被睾酮或其代谢物标记的细胞比例相同：腹侧超纹状体尾核（HVc）和新纹状体前部大细胞核（MAN）。这与斑胸草雀形成对比，在斑胸草雀中只有雄性唱歌：与雌性相比，HVc和MAN中雄性斑胸草雀被标记的细胞比例要高得多。这表明，发出复杂叫声的雌性鸟类已经进化出在类固醇激素敏感性方面与雄性极其相似的神经鸣叫控制系统。
