冷冻置换法能揭示微管中原纤维间结合的疏水性本质吗？

Does freeze-substitution reveal the hydrophobic nature of interprotofilament bonding in microtubules?

作者信息

Indi S, Wakley G, Stebbings H

出版信息

Cell Biol Int Rep. 1985 Sep;9(9):859-65. doi: 10.1016/0309-1651(85)90105-5.

Abstract

The massive system of microtubules in the ovarioles of Notonecta glauca has been prepared for electron microscopy by means of freeze-substitution. With this technique the protofilaments comprising the microtubule walls can be observed in positive image, indicating that the procedure provides a valuable method for observing microtubule substructure. Surprisingly though, the cryoprotectants, which are an integral part of the method, caused a rearrangement of the microtubules from their usual pattern in the ovarioles. These observations, we believe, provide an insight into intra- and intermicrotubule interactions respectively.

摘要

通过冷冻置换法制备了用于电子显微镜观察的黄仰蝽卵巢小管中的大量微管系统。利用该技术，可以在正像中观察到构成微管壁的原丝，这表明该方法为观察微管亚结构提供了一种有价值的方法。然而，令人惊讶的是，作为该方法不可或缺一部分的冷冻保护剂导致微管在卵巢小管中从其通常的模式发生了重排。我们认为，这些观察结果分别为微管内和微管间的相互作用提供了见解。