Wei Chunyin, Zheng Yanyan, Yan Fei, Xu Lifang
Guangxi Medical University Cancer Hospital, Guangxi Medical University, Nanning 530021, China.
Postdoctoral Research Center, School of Basic Medicine, Guangxi Medical University, Nanning 530021, China.
Biosensors (Basel). 2025 May 21;15(5):332. doi: 10.3390/bios15050332.
Glucose (Glu) detection, as a fundamental analytical technique, has applications in medical diagnostics, clinical testing, bioanalysis and environmental monitoring. In this work, a solid-phase electrochemiluminescence (ECL) enzyme sensor was developed by immobilizing the ECL emitter in a stable manner within bipolar silica nanochannel array film (bp-SNA), enabling sensitive glucose detection. The sensor was constructed using an electrochemical-assisted self-assembly (EASA) method with various siloxane precursors to quickly modify the surface of indium tin oxide (ITO) electrodes with a bilayer SNA of different charge properties. The inner layer, including negatively charged SNA (n-SNA), attracted the positively charged ECL emitter tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)) via electrostatic interaction, while the outer layer, including positively charged SNA (p-SNA), repelled it, forming a barrier that efficiently concentrated the Ru(bpy) emitter in a stable manner. After modifying the amine groups on the p-SNA surface with aldehyde groups, glucose oxidase (GOx) was covalently immobilized, forming the enzyme electrode. In the presence of glucose, GOx catalyzed the conversion of glucose to hydrogen peroxide (HO), which acted as a quencher for the Ru(bpy)/triethanolamine (TPA) system, reducing the ECL signal and enabling quantitative glucose analysis. The sensor exhibited a wide linear range from 10 μM to 7.0 mM and a limit of detection (LOD) of 1 μM (S/N = 3). Glucose detection in fetal bovine serum was realized. By replacing the enzyme type on the electrode surface, this sensing strategy holds the potential to provide a universal platform for the detection of different metabolites.
葡萄糖（Glu）检测作为一种基本的分析技术，在医学诊断、临床检测、生物分析和环境监测等领域有着广泛应用。在本研究中，通过将电化学发光（ECL）发光体以稳定的方式固定在双极二氧化硅纳米通道阵列膜（bp-SNA）中，开发了一种固相ECL酶传感器，实现了对葡萄糖的灵敏检测。该传感器采用电化学辅助自组装（EASA）方法，使用各种硅氧烷前驱体，快速用具有不同电荷性质的双层SNA修饰氧化铟锡（ITO）电极表面。内层包括带负电荷的SNA（n-SNA），通过静电相互作用吸引带正电荷的ECL发光体三（2,2'-联吡啶）钌（II）（Ru(bpy)），而外层包括带正电荷的SNA（p-SNA），将其排斥，形成一个屏障，有效地以稳定的方式浓缩Ru(bpy)发光体。在用醛基修饰p-SNA表面的胺基后，共价固定葡萄糖氧化酶（GOx），形成酶电极。在葡萄糖存在的情况下，GOx催化葡萄糖转化为过氧化氢（HO），过氧化氢作为Ru(bpy)/三乙醇胺（TPA）体系的猝灭剂，降低ECL信号，实现葡萄糖的定量分析。该传感器的线性范围为10 μM至7.0 mM，检测限（LOD）为1 μM（S/N = 3）。实现了胎牛血清中葡萄糖的检测。通过更换电极表面的酶类型，这种传感策略有潜力为不同代谢物的检测提供一个通用平台。
