Yuan Ximei, Meng Qingyao, Lu Zhengjie, Wang Chen, He Yiqing, Li Ke, Du Juan, Fu Yuejun
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi Key Laboratory of Biotechnology, Institute of Biotechnology, Shanxi University, Taiyuan, China.
Zhejiang Academy of Science & Technology for Inspection & Quarantine, Hangzhou, China.
Appl Environ Microbiol. 2025 Jun 18;91(6):e0061325. doi: 10.1128/aem.00613-25. Epub 2025 May 27.
multiple nucleopolyhedrovirus (AcMNPV) is the most widely studied baculovirus currently used as a biopesticide application for crop pest control. AcMNPV has a good control effect on Lepidoptera pests, but the impact of AcMNPV on other insects in the environment, especially natural enemies, is worth further discussion. Fallou, an essential natural enemy insect widely distributed in China, can prey on multiple pests that cause severe damage to the production economy and can play an effective role in preventing agroforestry pests. In this study, the natural enemy Fallou was used as the research material to analyze the effect of AcMNPV on the immune system of Fallou. The immune mechanism of AcMNPV-EGFP in infecting was revealed for the first time, and the critical factor miR-8 was found. The relationship between AcMNPV, miR-8, and was described. RNAi pathway and JAK/STAT signaling pathway are the main antiviral pathways in insects, and we have newly discovered the molecular regulation mechanism of the Toll signaling pathway in antiviral immunity. In a word, AcMNPV, a Lepidopteran insecticide, affects the immune response of Fallou and has side effects on natural enemies in the process of pest control.IMPORTANCEAcMNPV is a viral pesticide that is widely used to control Lepidoptera pests. However, the impact on other insects has yet to be thoroughly studied. Therefore, the toxicity and side effects of AcMNPV on non-target organisms need to be effectively evaluated. As a biological control agent in agricultural ecosystems, natural enemy insects can effectively control the number of pests and reduce crop damage. Compared with chemical pesticides, natural enemy insects can avoid or reduce pesticide residues in agricultural products and have no environmental pollution, and pests do not quickly develop resistance to them. It will help ensure crops' safe production and balance the ecosystem. In this study, AcMNPV-EGFP was used to infect the natural enemy- Fallou, which proved the effect of the virus pesticide on Fallou. This work helps optimize the joint use of the natural enemy- Fallou and other insecticides in applying biological control and also provides a reference for understanding the mechanism of insect antiviral infection.
多角体核型多角体病毒（AcMNPV）是目前研究最广泛的杆状病毒，被用作生物杀虫剂来防治农作物害虫。AcMNPV对鳞翅目害虫有良好的防治效果，但AcMNPV对环境中其他昆虫，尤其是天敌的影响值得进一步探讨。蠋蝽是一种在中国广泛分布的重要天敌昆虫，能捕食多种对生产经济造成严重损害的害虫，在防治农林害虫方面能发挥有效作用。本研究以天敌蠋蝽为研究材料，分析AcMNPV对蠋蝽免疫系统的影响。首次揭示了AcMNPV-EGFP感染蠋蝽的免疫机制，发现了关键因子miR-8。阐述了AcMNPV、miR-8与蠋蝽之间的关系。RNAi途径和JAK/STAT信号通路是昆虫主要的抗病毒途径，我们新发现了Toll信号通路在抗病毒免疫中的分子调控机制。总之，鳞翅目杀虫剂AcMNPV影响蠋蝽的免疫反应，在害虫防治过程中对天敌有副作用。
重要性
AcMNPV是一种广泛用于防治鳞翅目害虫的病毒杀虫剂。然而，其对其他昆虫的影响尚未得到充分研究。因此，需要有效评估AcMNPV对非靶标生物的毒性和副作用。天敌昆虫作为农业生态系统中的生物防治剂，能有效控制害虫数量，减少作物损害。与化学农药相比，天敌昆虫可避免或减少农产品中的农药残留，且无污染，害虫也不会迅速对其产生抗性。这将有助于确保作物安全生产并平衡生态系统。本研究中，用AcMNPV-EGFP感染天敌蠋蝽，证明了病毒杀虫剂对蠋蝽的作用。这项工作有助于优化天敌蠋蝽与其他杀虫剂在生物防治中的联合使用，也为理解昆虫抗病毒感染机制提供了参考。
