Huang Haisi, Qin Xiaojuan, Kang Yihao, Xu Jie, Shang Pengxiang, Chen Tingsu, Cheng Tong, Zhang Jinlian
Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
School of Public Health, Xiamen University, Xiamen 361102, China.
J Fungi (Basel). 2025 May 16;11(5):382. doi: 10.3390/jof11050382.
Arbuscular mycorrhizal (AM) fungi are root symbionts that play an important role in the growth of vascular plants. Four AM fungi, including three new species, , , , and a new country record from China, , are reported based on morphological characteristics and molecular phylogenetic analysis. They were isolated and propagated from spores extracted from the rhizosphere soils of citrus. . is characterized by forming hyaline to pale yellow globose to subglobose spores of (70.0-)85.0(-100.0) μm in diameter. Spores of are pale yellow to pale yellowish brown, with spherical to sub-spherical appearance and (103.1-)122.1(-147.1) μm in diameter. Young spores of are hyaline, gradually turning pale yellow as they mature, with spherical to sub-spherical appearance and (78.7-)85.6(-90.0) μm in diameter. Spores of are hyaline and 86.3-127.2 μm in diameter. Four species have three spore wall layers, and spores form individually in the soil. The phylogenetic tree was constructed and inferred from sequences of 18S-ITS1-5.8S-ITS2-28S datasets by Bayesian inference and maximum likelihood analysis. Voucher specimens are deposited in the Center for Subtropical Arbuscular Mycorrhizal Fungi Culture Collection (CSMC).
丛枝菌根（AM）真菌是根系共生体，在维管植物生长中发挥重要作用。基于形态特征和分子系统发育分析，报道了4种AM真菌，包括3个新物种，[物种名称1]、[物种名称2]、[物种名称3]，以及来自中国的1个新国家记录种[物种名称4]。它们是从柑橘根际土壤中提取的孢子分离和繁殖而来的。[物种名称1]的特征是形成直径为（70.0-）85.0（-100.0）μm的透明至浅黄色球形至近球形孢子。[物种名称2]的孢子为浅黄色至浅黄棕色，呈球形至近球形，直径为（103.1-）122.1（-147.1）μm。[物种名称3]的幼孢子是透明的，成熟时逐渐变为浅黄色，呈球形至近球形，直径为（78.7-）85.6（-90.0）μm。[物种名称4]的孢子是透明的，直径为86.3-127.2μm。这4个物种都有三层孢子壁，孢子在土壤中单独形成。通过贝叶斯推断和最大似然分析，根据18S-ITS1-5.8S-ITS2-28S数据集的序列构建并推断了系统发育树。凭证标本保存在亚热带丛枝菌根真菌培养物保藏中心（CSMC）。
