体内处理后小鼠肝细胞中肝细胞增殖和非常规DNA合成的诱导

Induction of hepatic cell proliferation and unscheduled DNA synthesis in mouse hepatocytes following in vivo treatment.

作者信息

Mirsalis J C, Tyson C K, Loh E N, Steinmetz K L, Bakke J P, Hamilton C M, Spak D K, Spalding J W

出版信息

Carcinogenesis. 1985 Oct;6(10):1521-4. doi: 10.1093/carcin/6.10.1521.

DOI:10.1093/carcin/6.10.1521

Abstract

We have modified the in vivo-in vitro hepatocyte DNA repair assay for measurement of unscheduled DNA synthesis (UDS) and hepatic cell proliferation in B6C3F1 mice. Dimethylnitrosamine and methylmethane sulfonate produced significant increases in UDS in both rats and mice. 2-Acetylaminofluorene induced a significant increase in UDS in rats, but not in mice. The mouse hepatocarcinogens, carbon tetrachloride, trichloroethylene, polybrominated biphenyls and 2,6-dichloro-p-phenylenediamine all failed to induce UDS in male and female mice, but all induced significant elevations in hepatic cell proliferation. Increased cell turnover in the liver may therefore be an important mechanism in hepatocarcinogenicity in the mouse.

摘要

我们已对体内-体外肝细胞DNA修复试验进行了改良，用于测量B6C3F1小鼠的DNA非预定合成（UDS）和肝细胞增殖。二甲基亚硝胺和甲基磺酸甲酯可使大鼠和小鼠的UDS显著增加。2-乙酰氨基芴可使大鼠的UDS显著增加，但对小鼠无此作用。小鼠致癌物四氯化碳、三氯乙烯、多溴联苯和2,6-二氯对苯二胺均未在雄性和雌性小鼠中诱导UDS，但均诱导肝细胞增殖显著升高。因此，肝脏中细胞更新增加可能是小鼠致癌作用的一个重要机制。