Griffiths W C, Lev R, Gentile J, Horvath G
Clin Biochem. 1985 Aug;18(4):254-6. doi: 10.1016/s0009-9120(85)80052-7.
Colonic and small intestinal alkaline phosphatase extracts were studied biochemically and electrophoretically to elucidate the source of a reported difference in cellulose acetate electrophoretic mobility. Both preparations were inactivated with 0.5 mmol/L L-phenylalanine but retained full activity in the presence of 1.0 mmol/L tetramisole. Treatment with neuraminidase changed a minor fraction of the small intestinal but the major portion of the colonic alkaline phosphatase to a cathodically migrating form. The most likely explanation for our findings is that the colon and small intestinal alkaline phosphatase are mixtures of the same multiple forms but in different proportions.
对结肠和小肠碱性磷酸酶提取物进行了生化和电泳研究，以阐明所报道的醋酸纤维素电泳迁移率差异的来源。两种制剂都被0.5 mmol/L的L-苯丙氨酸灭活，但在1.0 mmol/L四咪唑存在下保持全部活性。用神经氨酸酶处理后，小肠碱性磷酸酶的一小部分和结肠碱性磷酸酶的大部分转变为向阴极迁移的形式。对我们研究结果最可能的解释是，结肠和小肠碱性磷酸酶是相同多种形式的混合物，但比例不同。
