Liu Huabin, Bai Chuangyang, Cai Jiahui, Wu Yue, Zhu Changwei
College of Biomedicine and Health, Anhui Science and Technology University, Chuzhou 233100, China.
College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China.
Toxics. 2025 May 17;13(5):406. doi: 10.3390/toxics13050406.
The phytotoxicity of aluminum (Al) to plants is well known. Auxin accumulation and reactive oxygen species (ROS) burst induced by Al toxicity are the key factors in root growth inhibition. Yucasin, an auxin synthesis inhibitor, effectively ameliorates Al phytotoxicity in tomato seedlings. However, the physiological mechanisms by which yucasin alleviates Al phytotoxicity in tomatoes remain elusive. Here, we examined the regulatory mechanisms of yucasin involved in tomato seedling growth under Al conditions through phenotypic, plant physiology analysis, and cellular experiments. Exogenous indole-3-acetic acid (IAA) application increased Al accumulation in tomato seedling roots, while yucasin decreased Al accumulation. Yucasin application reduced Al-induced ROS accumulation, lipid peroxidation, and cell death, enhanced root viability, and promoted tomato seedling root growth. Further, yucasin enhanced the antioxidant enzyme activities of superoxide dismutase, catalase, and peroxidase in plants under Al conditions. The results suggest that yucasin improves the scavenging capacity of ROS by maintaining the activities of antioxidative enzymes. This study elucidates the physiological mechanism by which yucasin alleviates Al phytotoxicity, highlighting its potential to enhance plant tolerance under acidic Al conditions.
铝（Al）对植物的植物毒性是众所周知的。铝毒性诱导的生长素积累和活性氧（ROS）爆发是抑制根系生长的关键因素。玉卡辛是一种生长素合成抑制剂，可有效减轻番茄幼苗的铝植物毒性。然而，玉卡辛减轻番茄铝植物毒性的生理机制仍不清楚。在此，我们通过表型、植物生理学分析和细胞实验，研究了玉卡辛在铝胁迫条件下参与番茄幼苗生长的调控机制。外源施加吲哚-3-乙酸（IAA）增加了番茄幼苗根系中的铝积累，而玉卡辛则降低了铝积累。施用玉卡辛减少了铝诱导的ROS积累、脂质过氧化和细胞死亡，提高了根系活力，促进了番茄幼苗根系生长。此外，玉卡辛增强了铝胁迫条件下植物中超氧化物歧化酶、过氧化氢酶和过氧化物酶的抗氧化酶活性。结果表明，玉卡辛通过维持抗氧化酶的活性提高了ROS的清除能力。本研究阐明了玉卡辛减轻铝植物毒性的生理机制，突出了其在酸性铝条件下增强植物耐受性的潜力。
