Praseetha P K, Vijayakumar S, Gangadhar Lekshmi, Gopukumar S T, Vijayakumar S
Department of Nanotechnology, Noorul Islam Centre for Higher Education, Kumaracoil, Tamilnadu, India.
Department of Botany, A.V.V.M Sri Pushpam College (Autonomous) Affiliated to Bharathidasan University, Poondi, Thanjavur, Tamil Nadu, India.
Appl Biochem Biotechnol. 2025 May 27. doi: 10.1007/s12010-025-05282-5.
The health of millions of people is seriously threatened by infectious diseases that spread rapidly within communities and can lead to outbreaks if not effectively controlled by medical personnel. This study examines the complex mechanisms of antibiotic resistance, specifically focusing on the emergence of methicillin-resistant Staphylococcus aureus (MRSA) and Extended-Spectrum Beta-Lactamase (ESBL)-producing bacteria in Indian healthcare settings. MRSA isolates exhibited complete resistance to ampicillin, ciprofloxacin, amoxicillin, and amoxicillin-clavulanic acid on Mueller-Hinton agar plates. Characterization results indicated an increased inhibition zone diameter and enhanced encapsulation integrity. UV-visible spectrophotometric analysis revealed that ciprofloxacin-loaded liposomes achieved an entrapment efficiency of 16.45% after 1 h, increasing to 76% after 24 h. Encapsulation of ciprofloxacin, amikacin, cloxacillin, and vancomycin within vesicles demonstrated improved antimicrobial efficacy against Escherichia coli, Staphylococcus aureus, Acinetobacter baumannii, Klebsiella pneumoniae, and MRSA. Moreover, liposome-encapsulated aminoglycosides exhibited promising potential against A. baumannii, particularly in localized infections where sustained drug concentrations at the infection site are essential. The results of this study suggest that liposomal antibiotics hold significant potential for treating severe infections both systemically and topically. They may enhance therapeutic effectiveness while minimizing adverse effects, offering a promising approach to combating antibiotic-resistant bacterial infections.
数百万人的健康受到传染病的严重威胁，这些传染病在社区内迅速传播，如果医务人员不能有效控制，可能导致疫情爆发。本研究考察了抗生素耐药性的复杂机制，特别关注印度医疗环境中耐甲氧西林金黄色葡萄球菌（MRSA）和产超广谱β-内酰胺酶（ESBL）细菌的出现。在穆勒-欣顿琼脂平板上，MRSA分离株对氨苄西林、环丙沙星、阿莫西林和阿莫西林-克拉维酸表现出完全耐药性。表征结果表明抑菌圈直径增加，包封完整性增强。紫外-可见分光光度分析显示，载有环丙沙星的脂质体在1小时后包封率为16.45%，24小时后增至76%。将环丙沙星、阿米卡星、氯唑西林和万古霉素包裹在囊泡中，对大肠杆菌、金黄色葡萄球菌、鲍曼不动杆菌、肺炎克雷伯菌和MRSA显示出更好的抗菌效果。此外，脂质体包裹的氨基糖苷类药物对鲍曼不动杆菌显示出有前景的潜力，特别是在局部感染中，在感染部位维持药物浓度至关重要。本研究结果表明，脂质体抗生素在全身和局部治疗严重感染方面具有巨大潜力。它们可能提高治疗效果，同时将副作用降至最低，为对抗耐药细菌感染提供了一种有前景的方法。
