Yang Ying, Pu Qiuhong, Lv Yonggang, Li Jing, Zhou Jiawu, Deng Xianneng, Song Xuanchen, Zhang Yu, Tao Dayun
Yunnan Seed Laboratory, Yunnan Key Laboratory for Rice Genetic Improvement, Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS), Kunming, 650200, China.
Rice (N Y). 2025 May 27;18(1):43. doi: 10.1186/s12284-025-00803-6.
Hybrid sterility is a common phenomenon in hybrids between the Asian cultivated rice (Oryza sativa L.) and its relatives with AA genome, which limits the utilization of interspecific heterosis and favorable gene introgression. Numerous loci for hybrid sterility have been identified between O. sativa and its relatives. However, it remains elusive whether hybrid sterility between different species is controlled by a set of conserved loci, and whether there are variations in the genetic mode of these loci.
In this study, six novel hybrid sterility loci for pollen sterility were identified from different cross combinations between O. sativa and its three wild relatives. S59 caused hybrid pollen sterility in hybrids between O. sativa and O. rufipogon. S60 and S61 controlled the hybrid pollen sterility between O. sativa and O. glumaepatula. S62, S63 and S64 governed the hybrid pollen sterility between O. sativa and O. barthii. Genetic and linkage analysis showed that S59, S60, and S62 were located in near the same region on the short arm of chromosome 5. S61 and S63 were mapped near RM27460 on the short arm of chromosome 12. S64 was restricted into the 60.27 kb region between RM4853 and RM3372 on the short arm of chromosome 3. The genetic behavior of six novel hybrid sterility loci follows one-locus allelic interaction model, the male gametes carrying the alleles of O. sativa in the heterozygotes were selectively aborted except for S62.
The findings from this research would provide a better understanding for the genetic nature of interspecific hybrid sterility in rice.
杂种不育是亚洲栽培稻（Oryza sativa L.）与其具有AA基因组的近缘种之间杂交的常见现象，这限制了种间杂种优势的利用和有利基因的渗入。在栽培稻与其近缘种之间已鉴定出许多杂种不育位点。然而，不同物种间的杂种不育是否由一组保守位点控制，以及这些位点的遗传模式是否存在变异，仍不清楚。
在本研究中，从栽培稻与其三个野生近缘种的不同杂交组合中鉴定出六个新的花粉不育杂种不育位点。S59导致栽培稻与野生稻杂种的花粉不育。S60和S61控制栽培稻与阔叶野生稻之间的杂种花粉不育。S62、S63和S64决定栽培稻与短药野生稻之间的杂种花粉不育。遗传和连锁分析表明，S59、S60和S62位于第5号染色体短臂的同一区域附近。S61和S63定位于第12号染色体短臂的RM27460附近。S64被定位在第3号染色体短臂上RM4853和RM3372之间60.27 kb的区域内。六个新的杂种不育位点的遗传行为遵循一位点等位基因互作模型，杂合子中携带栽培稻等位基因的雄配子除S62外均被选择性淘汰。
本研究结果将有助于更好地理解水稻种间杂种不育的遗传本质。
