Li Jing, Zhou Jiawu, Xu Peng, Deng Xianneng, Deng Wei, Zhang Yu, Yang Ying, Tao Dayun
Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS), Kunming 650200, P. R. China.
Breed Sci. 2018 Dec;68(5):516-523. doi: 10.1270/jsbbs.18001. Epub 2018 Nov 16.
is a potential source for improving Asian cultivated rice via direct hybridization and backcrossing. However, hybrid sterility between and is the main barrier of reproduction hindering the transfer of favorable genes from to . To investigate the nature of hybrid sterility between and , three accessions of were used as male parents to cross Dianjingyou 1, an subsp. cultivar following the backcross with the recurrent parent of Dianjingyou 1. Twenty pollen sterility NILs (BCF) were obtained and genotyped by using simple sequence repeat (SSR) markers distributed across the 12 rice chromosomes. The heterozygous markers were employed to genotype the corresponding segregation populations for mapping sterility genes. As a result, five novel loci for pollen sterility between and were identified and designated as (t), (t), (t), (t) and (t), respectively. The genetic behavior of five novel loci followed one-locus allelic interaction model. The disharmonious interaction between Asian cultivated rice allele and wild relative allele led to the partial or full abortion of male gametes for one parent allele in the heterozygotes. These results will be useful for elucidating the mechanism of interspecific hybrid sterility and further utilizing favorable genes from for enhancement of rice breeding.
是通过直接杂交和回交改良亚洲栽培稻的潜在基因源。然而，与之间的杂种不育是生殖的主要障碍，阻碍了有利基因从向的转移。为了研究与之间杂种不育的本质，以三个的种质作为父本与滇粳优1（一个亚种栽培品种）杂交，随后与滇粳优1的轮回亲本回交。获得了20个花粉不育近等基因系（BCF），并使用分布在12条水稻染色体上的简单序列重复（SSR）标记进行基因分型。利用杂合标记对相应的分离群体进行基因分型以定位不育基因。结果，鉴定出了与之间花粉不育的5个新位点，分别命名为（t）、（t）、（t）、（t）和（t）。5个新位点的遗传行为遵循单基因座等位基因互作模型。亚洲栽培稻等位基因与野生近缘种等位基因之间的不协调互作导致杂合子中一个亲本等位基因的雄配子部分或完全败育。这些结果将有助于阐明种间杂种不育的机制，并进一步利用来自的有利基因来加强水稻育种。
