氨基酸转运的抗原特异性免疫刺激：牛结核病诊断的建议方法。
Antigen-specific immunostimulation of amino acid transport: proposed method for the diagnosis of tuberculosis in cattle.
作者信息
Tate E H, Cram L S
出版信息
Diagn Immunol. 1985;3(2):89-96.
Preliminary data on a novel method for the diagnosis of bovine tuberculosis (TB) has been presented. This method is based on increased amino acid transport in specifically stimulated lymphocyte cultures. Lymphocytes isolated from TB-infected or -sensitized cattle and cultured with the TB antigen PPD-B had noticeably enhanced amino acid transport after 1-3 days. Transport continued to increase for up to 5 days of culture with the antigen. PPD-B did not stimulate transport in lymphocytes from nonsensitized animals. The new method correlated well with the tuberculin skin test and antigen-induced lymphocyte proliferation measured by flow cytometry.
一种诊断牛结核病（TB）的新方法的初步数据已经公布。该方法基于在特异性刺激的淋巴细胞培养物中氨基酸转运增加。从感染或致敏结核病的牛中分离出淋巴细胞，并用结核菌素抗原PPD-B培养，1-3天后氨基酸转运显著增强。在与抗原培养长达5天的过程中，转运持续增加。PPD-B不会刺激未致敏动物淋巴细胞的转运。这种新方法与结核菌素皮肤试验以及通过流式细胞术测量的抗原诱导的淋巴细胞增殖密切相关。
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