Wood P R, Jones S L
Research and Development, CSL Animal Health, 45 Poplar Road, Parkville, Victoria, Australia.
Tuberculosis (Edinb). 2001;81(1-2):147-55. doi: 10.1054/tube.2000.0272.
BOVIGAM which is based on the detection of gamma interferon (IFN- gamma) is a rapid, laboratory assay of a cell mediated immune response that may be used for the detection of tuberculosis (TB) infection in animals. Whole blood is first incubated overnight with bovine PPD, avian PPD or negative control antigens, and IFN- gamma in the supernatant plasma is then measured by EIA. TB infection is indicated by a predominant IFN- gamma response to bovine PPD. Since 1988, BOVIGAM has been extensively trialed on more than 200 000 cattle in Australia, Brazil, Ireland, Northern Ireland, Italy, New Zealand, Romania, Spain and the USA. Sensitivity has varied between 81.8% and 100% for culture-confirmed bovine TB and specificity between 94% and 100%. The IFN- gamma assay detects M. bovis infection earlier than the skin test and in New Zealand is applied to detect skin-test negative cattle with TB, where after slaughter a significant number of IFN- gamma reactors have TB. BOVIGAM is also approved in New Zealand for serial testing skin test positive cattle when non-specificity is suspected. Cattle are tested 7-30 days after a positive caudal fold test. The boosting effect of the skin test on T-cell activity allows blood to be cultured with PPD up to 30 h after collection without effecting accuracy. The BOVIGAM results are not affected by poor nutritional condition and are only mildly and briefly affected by dexamethasone treatment and parturition. IFN- gamma responses of cattle vaccinated with BCG are dose-dependent and short-lived. The BOVIGAM kit is now used routinely in many countries for the detection of M. bovis infected cattle, buffalo and goats.
基于γ干扰素（IFN-γ）检测的BOVIGAM是一种快速的细胞介导免疫反应实验室检测方法，可用于检测动物的结核病（TB）感染。首先将全血与牛型结核菌素纯蛋白衍生物（PPD）、禽型PPD或阴性对照抗原孵育过夜，然后通过酶免疫分析（EIA）测定上清血浆中的IFN-γ。对牛型PPD的主要IFN-γ反应表明存在TB感染。自1988年以来，BOVIGAM已在澳大利亚、巴西、爱尔兰、北爱尔兰、意大利、新西兰、罗马尼亚、西班牙和美国的20多万头牛身上进行了广泛试验。对于经培养确诊的牛结核病，敏感性在81.8%至100%之间，特异性在94%至100%之间。IFN-γ检测比皮肤试验更早检测到牛分枝杆菌感染，在新西兰，该检测用于检测皮肤试验阴性但患有结核病的牛，屠宰后发现大量IFN-γ反应阳性的牛患有结核病。在新西兰，当怀疑存在非特异性时，BOVIGAM也被批准用于对皮肤试验阳性的牛进行系列检测。在尾褶试验呈阳性后7至30天对牛进行检测。皮肤试验对T细胞活性的增强作用使得血液在采集后长达30小时内都可以与PPD一起培养而不影响准确性。BOVIGAM的结果不受营养状况不佳的影响，仅受到地塞米松治疗和分娩的轻微且短暂的影响。接种卡介苗的牛的IFN-γ反应具有剂量依赖性且持续时间短。目前，BOVIGAM试剂盒在许多国家被常规用于检测感染牛分枝杆菌的牛、水牛和山羊。
