Hall F, Tengerdy C, Morita M, Pautler E
Curr Eye Res. 1985 Aug;4(8):847-50. doi: 10.3109/02713688509095251.
Bovine retinae, devoid of pigment epithelium, were assayed for endogenous melatonin using high-performance liquid chromatography with electrochemical detection (HPLC-EC). A method of extracting the more polar biogenic amines and metabolites was employed, allowing for direct injection of retinal supernatants into the chromatographic column.
使用高效液相色谱-电化学检测法(HPLC-EC)对去除色素上皮的牛视网膜进行内源性褪黑素检测。采用了一种提取极性更强的生物胺和代谢物的方法,可将视网膜上清液直接注入色谱柱。
1)证实在牛视网膜中存在褪黑素;2)为光期收集的正常成年视网膜提供了3.01纳克/视网膜的“基准”;3)表明HPLC-EC是检测和定量单个牛视网膜中褪黑素的足够灵敏的仪器。