Buzney S M, Frank R N, Varma S D, Tanishima T, Gabbay K H
Invest Ophthalmol Vis Sci. 1977 May;16(5):392-6.
Cultured mural cells (intramural pericytes) from adult rhesus retinal capillaries were examined for the presence of the sorbitol pathway. A radioimmunoassay for human aldose reductase, cross-reactive with rhesus lens aldose reductase, showed the presence of this enzyme in our cultured cells. Mural cells grown in culture media containing normal (10 mM) and high (40 mM) levels of glucose were examined for polyol accumulation by gas-liquid chromatography. Cells incubated in high glucose medium showed a threefold increase in sorbitol concentration over cells grown at low glucose levels. After 30 days in high glucose medium, mural cells formed dense multilayered areas with extensive cellular debris. These findings suggest the presence of the sorbitol pathway in cultured retinal mural cells and cellular degeneration in high glucose medium; this may have possible implications in the pathogenesis of diabetic retinopathy.
对成年恒河猴视网膜毛细血管的培养壁细胞（壁内周细胞）进行了山梨醇途径的检测。一种与人醛糖还原酶交叉反应的恒河猴晶状体醛糖还原酶放射免疫测定法显示，在我们培养的细胞中存在这种酶。通过气液色谱法检测在含有正常（10 mM）和高（40 mM）葡萄糖水平的培养基中生长的壁细胞的多元醇积累情况。在高葡萄糖培养基中孵育的细胞显示，其山梨醇浓度比在低葡萄糖水平下生长的细胞增加了三倍。在高葡萄糖培养基中培养30天后，壁细胞形成了带有大量细胞碎片的致密多层区域。这些发现表明，培养的视网膜壁细胞中存在山梨醇途径，且在高葡萄糖培养基中细胞会发生变性；这可能对糖尿病视网膜病变的发病机制具有潜在影响。
