Báez-Díaz Claudia, Torrescusa-Bermejo Axiel, Sánchez-Margallo Francisco Miguel, Vázquez-López Fátima, Pulido María, López Esther, Arenal Ángel, Crisóstomo Verónica
Fundación Centro de Cirugía de Mínima Invasión Jesús Usón, 10071 Cáceres, Spain.
Red Española de Terapias Avanzadas RICORS-TERAV, 28029 Madrid, Spain.
Biomedicines. 2025 Apr 25;13(5):1043. doi: 10.3390/biomedicines13051043.
Ventricular tachycardia (VT) resulting in sudden cardiac death is common following a myocardial infarction (MI). Our objective was to evaluate the effects of an intracoronary (IC) administration of cardiosphere-derived cell secretome (S-CDCs) on VT inducibility and cardiac function in a swine model of MI. Fourteen pigs underwent endovascular MI model creation. At 4 weeks, saline (CON; 5 mL; n = 7) or S-CDCs (S-CDCs; 9.16 mg protein in 5 mL saline; n = 7) was blindly administered via the IC route. VT inducibility and magnetic resonance imaging (MRI) studies were performed both pre- and 4 months post-IC therapy, calculating left ventricular ejection fraction (LVEF), infarct size as a percentage of left ventricle (% MI), and left ventricular indexed end-diastolic and end-systolic volumes (LVEDVi, LVESVi). While VT was inducible in 100% of the animals before IC therapy, at 4 months, the inducibility rate was lower in the S-CDCs group compared to the CON group (57% versus 100%, = 0.05). Likewise, in the S-CDCs group, % MI was significantly lower than in the CON group (12 ± 3% versus 16 ± 3%, = 0.03). LVEF (S-CDCs: 35 ± 10% versus CON: 29 ± 10%, = NS), LVEDVi and LVESVi (S-CDCs: 83 ± 18 mL/m and 56 ± 20 mL/m versus CON: 88 ± 29 mL/m and 64 ± 20 mL/m, = NS) did not change. IC therapy with S-CDCs appears to reduce the development of post-MI VT. Furthermore, it suggests a beneficial effect on infarct size, reducing % MI in this experimental swine model.
心肌梗死后，室性心动过速（VT）导致的心源性猝死很常见。我们的目的是评估在猪心肌梗死模型中，冠状动脉内（IC）注射心肌球衍生细胞分泌组（S-CDCs）对VT诱发性和心脏功能的影响。14头猪接受了血管内心肌梗死模型创建。4周时，通过IC途径盲目注射生理盐水（CON组；5 mL；n = 7）或S-CDCs（S-CDCs组；5 mL生理盐水中含9.16 mg蛋白质；n = 7）。在IC治疗前和治疗后4个月进行VT诱发性和磁共振成像（MRI）研究，计算左心室射血分数（LVEF）、梗死面积占左心室的百分比（% MI）以及左心室指数舒张末期和收缩末期容积（LVEDVi、LVESVi）。在IC治疗前，所有动物均能诱发VT，但在4个月时，S-CDCs组的诱发率低于CON组（57%对100%，P = 0.05）。同样，S-CDCs组的% MI显著低于CON组（12±3%对16±3%，P = 0.03）。LVEF（S-CDCs组：35±10%对CON组：29±10%，P = 无显著性差异）、LVEDVi和LVESVi（S-CDCs组：83±18 mL/m和56±20 mL/m对CON组：88±29 mL/m和64±20 mL/m，P = 无显著性差异）没有变化。IC注射S-CDCs治疗似乎可减少心肌梗死后VT的发生。此外，这表明对梗死面积有有益影响，在该实验猪模型中可降低% MI。
