益生菌作为抗肿瘤剂：来自女性肿瘤细胞培养研究的见解

Probiotics as Anti-Tumor Agents: Insights from Female Tumor Cell Culture Studies.

作者信息

Giorgi Chiara, Lombardi Francesca, Augello Francesca Rosaria, Alicka Ylli, Quintiliani Massimiliano, Topi Skender, Cimini Annamaria, Castelli Vanessa, d'Angelo Michele

机构信息

Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy.

Department of Clinical Disciplines, University "Alexander Xhuvani" of Elbasan, 3001 Elbasan, Albania.

出版信息

Biomolecules. 2025 May 2;15(5):657. doi: 10.3390/biom15050657.

DOI:10.3390/biom15050657

PMID:40427550

Abstract

Breast and ovarian cancers are among the most prevalent cancers in women. Cancerous cells are characterized by their ability to continuously cycle and migrate, forming metastases. Some probiotic strains have shown anti-tumorigenic effects. This study tested the impact of probiotics on OVCAR-3 and MDA-MB-231 cell lines by analyzing proteins involved in cell cycle regulation (pP53, Cyclin D1, pERK1), cell survival (AKT), and cell migration (RhoA) using Western blotting and scratch wound tests. Results indicated a reduction in these proteins and decreased cell migration velocity post-treatment. These findings suggest that certain probiotic combinations can arrest the cell cycle, promote cell death, and reduce cell migration, potentially serving as promising candidates alongside standard therapies.

摘要

乳腺癌和卵巢癌是女性中最常见的癌症之一。癌细胞的特征在于其持续循环和迁移并形成转移灶的能力。一些益生菌菌株已显示出抗肿瘤作用。本研究通过蛋白质免疫印迹法和划痕试验分析参与细胞周期调控（pP53、细胞周期蛋白D1、pERK1）、细胞存活（AKT）和细胞迁移（RhoA）的蛋白质，测试了益生菌对OVCAR-3和MDA-MB-231细胞系的影响。结果表明，处理后这些蛋白质减少，细胞迁移速度降低。这些发现表明，某些益生菌组合可以使细胞周期停滞，促进细胞死亡并减少细胞迁移，有可能成为标准疗法之外有前景的候选方案。