Díaz-Del Cerro Estefanía, Félix Judith, Martínez-Poyato Mª Carmen, De la Fuente Mónica
Department of Genetics, Physiology and Microbiology (Unity of Animal Physiology), Faculty of Biological Science, Complutense University of Madrid (UCM), 28040 Madrid, Spain.
Institute of Investigation 12 de Octubre (imas12), 28041 Madrid, Spain.
Biomolecules. 2025 May 20;15(5):739. doi: 10.3390/biom15050739.
Aging involves immune system deterioration (immunosenescence) and increased oxidative stress, both associated with morbidity and mortality. Menopause accelerates aging, highlighting the need for strategies to mitigate its effects in postmenopausal women. This study assessed the impact of daily oral supplementation for one month with 39 bioactive compounds (UNAMINA)-including amino acids, vitamins, and antioxidants-on immune function, redox parameters, stress-related hormones, and biological age in healthy postmenopausal women. Peripheral blood samples were collected before and after supplementation to analyze lymphocyte and neutrophil functions (adherence, chemotaxis, natural killer cell antitumor capacity, and lymphoproliferative response to mitogens), oxidative stress markers (antioxidant defenses such as glutathione peroxidase (GPx) and reductase activities, reduced glutathione (GSH) concentrations, as well as oxidants such as oxidized glutathione (GSSG), and lipid peroxidative damage) in blood cells, and stress-related hormones (dehydroepiandrosterone (DHEA) and cortisol) in plasma. Supplementation improved all immune cell functions and decreased oxidative stress (increasing antioxidants defenses such as GPx activity and GSH concentration and decreasing GSSG amount) and cortisol concentrations, whereas those of DHEA increased. The biological age also decreased. The results suggest that these bioactive compounds may be a beneficial strategy for promoting healthier aging in postmenopausal women by enhancing immune function, reducing biological age, improving redox balance, and regulating stress hormones.
衰老涉及免疫系统衰退（免疫衰老）和氧化应激增加，二者均与发病率和死亡率相关。更年期会加速衰老，这凸显了采取策略减轻其对绝经后女性影响的必要性。本研究评估了连续一个月每日口服包含39种生物活性化合物（UNAMINA）——包括氨基酸、维生素和抗氧化剂——对健康绝经后女性免疫功能、氧化还原参数、应激相关激素和生物学年龄的影响。在补充前后采集外周血样本，以分析淋巴细胞和中性粒细胞功能（黏附、趋化性、自然杀伤细胞抗肿瘤能力以及对有丝分裂原的淋巴细胞增殖反应）、血细胞中的氧化应激标志物（抗氧化防御，如谷胱甘肽过氧化物酶（GPx）和还原酶活性、还原型谷胱甘肽（GSH）浓度，以及氧化剂，如氧化型谷胱甘肽（GSSG）和脂质过氧化损伤），以及血浆中的应激相关激素（脱氢表雄酮（DHEA）和皮质醇）。补充剂改善了所有免疫细胞功能，降低了氧化应激（增加了诸如GPx活性和GSH浓度等抗氧化防御，并减少了GSSG量）以及皮质醇浓度，而DHEA浓度增加。生物学年龄也有所下降。结果表明，这些生物活性化合物可能是一种有益的策略，可通过增强免疫功能、降低生物学年龄、改善氧化还原平衡和调节应激激素，促进绝经后女性更健康地衰老。
