姜黄挥发油及其纳米制剂的抗癌活性：细胞毒性、细胞凋亡和细胞迁移作用。
Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects.
机构信息
Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.
Cancer Research Unit of Associated Medical Sciences (AMS CRU), Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.
出版信息
BMC Complement Med Ther. 2024 Jan 2;24(1):16. doi: 10.1186/s12906-023-04261-9.
BACKGROUND AND AIMS
Curcuma aeruginosa, commonly known as "kha-min-dam" in Thai, holds significance in Asian traditional medicine due to its potential in treating various diseases, having properties such as anti-HIV, hepatoprotective, antimicrobial and anti-androgenic activities. This study explores the anticancer activity of C. aeruginosa essential oil (CAEO) and its nano-formulations.
METHODS
CAEO obtained from hydrodistillation of C. aeruginosa fresh rhizomes was examined by gas chromatography mass spectroscopy. Cytotoxicity of CAEO was determined in leukaemic K562 and breast cancer MCF-7 cell lines using an MTT assay. Cell cycle analysis and cell apoptosis were determined by flow cytometry. Cell migration was studied through a wound-healing assay.
RESULTS
Benzofuran (33.20%) emerged as the major compound of CAEO, followed by Germacrene B (19.12%) and Germacrone (13.60%). Two types of CAEO loaded nano-formulations, nanoemulsion (NE) and microemulsion (ME) were developed. The average droplet sizes of NE and ME were 13.8 ± 0.2 and 21.2 ± 0.2 nm, respectively. In a comparison with other essential oils from the fresh rhizomes of potential plants from the same family (Curcuma longa, Curcuma mangga and Zingiber officinale) on anticancer activity against K562 and MCF-7 cell lines, CAEO exhibited the highest cytotoxicity with IC of 13.43 ± 1.09 and 20.18 ± 1.20 µg/mL, respectively. Flow cytometry analysis revealed that CAEO significantly increased cell death, evidenced from the sub-G1 populations in the cell cycle assay and triggered apoptosis. Additionally, CAEO effectively inhibited cell migration in MCF-7 cells after incubation for 12 and 24 h. The developed NE and ME formulations significantly enhanced the cytotoxicity of CAEO against K562 cells with an IC of 45.30 ± 1.49 and 41.98 ± 0.96 µg/mL, respectively.
CONCLUSION
This study's finding suggest that both nano-formulations, NE and ME, effectively facilitated the delivery of CAEO into cancer cells.
背景与目的
姜黄属植物在亚洲传统医学中具有重要地位，被称为“kha-min-dam”，因其具有抗 HIV、保肝、抗菌和抗雄激素等特性，可用于治疗各种疾病。本研究旨在探索姜黄属植物精油（CAEO）及其纳米制剂的抗癌活性。
方法
采用气相色谱-质谱联用技术对姜黄属植物新鲜根茎水蒸馏得到的 CAEO 进行分析。采用 MTT 法测定 CAEO 在白血病 K562 和乳腺癌 MCF-7 细胞系中的细胞毒性。通过流式细胞术测定细胞周期分析和细胞凋亡。通过划痕愈合试验研究细胞迁移。
结果
CAEO 的主要化合物为苯并呋喃（33.20%），其次为大根香叶烯 B（19.12%）和姜黄烯（13.60%）。开发了两种 CAEO 负载的纳米制剂，即纳米乳液（NE）和微乳液（ME）。NE 和 ME 的平均粒径分别为 13.8 ± 0.2nm 和 21.2 ± 0.2nm。与同一科（姜黄、芒果姜黄和生姜）潜在植物新鲜根茎的其他精油在抗癌活性方面进行比较，CAEO 对 K562 和 MCF-7 细胞系的细胞毒性最高，IC 分别为 13.43 ± 1.09µg/mL 和 20.18 ± 1.20µg/mL。流式细胞术分析表明，CAEO 显著增加了细胞死亡，表现在细胞周期检测中的亚 G1 群体和触发的细胞凋亡。此外，CAEO 能有效抑制 MCF-7 细胞的迁移，孵育 12 和 24 小时后细胞迁移减少。开发的 NE 和 ME 制剂显著提高了 CAEO 对 K562 细胞的细胞毒性，IC 分别为 45.30 ± 1.49µg/mL 和 41.98 ± 0.96µg/mL。
结论
本研究结果表明，纳米制剂 NE 和 ME 均能有效促进 CAEO 进入癌细胞。
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