Chai Wenbo, Yuan Chao, Li Shufen, Xu Hanyuan, Zhu Qing, Li Hongtao, Ji Wei, Wang Jun
Lianyungang Academy of Agricultural Sciences, Lianyungang 222006, China.
Biology (Basel). 2025 May 6;14(5):508. doi: 10.3390/biology14050508.
The Di19 (Drought-induced 19) gene family encodes Cys2/His2-type zinc finger proteins that are known to be involved in plant responses to various abiotic stresses, including drought, salinity, and temperature extremes. However, little is known about their roles in barley (), particularly in cold stress adaptation. This study aimed to conduct a comprehensive genome-wide analysis of the barley genome to identify Di19 gene family members and examine their expression patterns under cold stress, providing theoretical support for stress-resistant barley breeding. By aligning Di19 gene sequences from and rice and using BLASTp, seven genes were identified in barley. Bioinformatics analysis revealed that all members contain a conserved Cys2/His2-type zinc finger domain and nuclear localization signals. Phylogenetic analysis grouped the genes into four subfamilies, with three homologous gene pairs, and Ka/Ks analysis indicated strong purifying selection. Tissue-specific expression analysis showed significant variation in expression across barley organs. Under cold stress, different barley varieties exhibited distinct gene expression profiles: for instance, was downregulated in cold-tolerant varieties, whereas showed increased expression in a cold-tolerant mutant, suggesting their potential roles in modulating cold response. These findings reveal the evolutionary conservation and cold-responsive expression characteristics of the gene family, laying a foundation for future functional studies. The results also provide important molecular resources for the genetic improvement of cold tolerance in barley, contributing to the development of stress-resilient crop varieties under climate change.
Di19（干旱诱导19）基因家族编码Cys2/His2型锌指蛋白，已知其参与植物对各种非生物胁迫的响应，包括干旱、盐胁迫和极端温度。然而，它们在大麦中的作用，特别是在适应冷胁迫方面，人们了解甚少。本研究旨在对大麦基因组进行全面的全基因组分析，以鉴定Di19基因家族成员并检测它们在冷胁迫下的表达模式，为抗逆大麦育种提供理论支持。通过比对拟南芥和水稻的Di19基因序列并使用BLASTp，在大麦中鉴定出7个Di19基因。生物信息学分析表明，所有成员都含有保守的Cys2/His2型锌指结构域和核定位信号。系统发育分析将Di19基因分为四个亚家族，有三对同源基因对，Ka/Ks分析表明存在强烈的纯化选择。组织特异性表达分析显示，Di19基因在大麦各器官中的表达存在显著差异。在冷胁迫下，不同大麦品种表现出不同的Di19基因表达谱：例如，Di19在耐寒品种中下调，而在一个耐寒突变体中表达增加，表明它们在调节冷响应中可能发挥的作用。这些发现揭示了Di19基因家族的进化保守性和冷响应表达特征，为未来的功能研究奠定了基础。研究结果还为大麦耐寒性的遗传改良提供了重要的分子资源，有助于在气候变化下培育抗逆作物品种。
